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Pseudomonas pachastrellae sp. nov., isolated from a marine sponge

¹ Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospect 100 Let Vladivostoku, 159, Russia
² Laboratory of Food Science and Technology, Department of Applied Biology and Chemistry, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
³ CCUG Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Guldhedsgatan 10, S-413 46 Göteborg, Sweden
⁴ Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

Correspondence
Lyudmila A. Romanenko
lro{at}piboc.dvo.ru

Two Gram-negative, non-fermentative, non-denitrifying, non-pigmented, rod-shaped bacteria that were motile by means of polar flagella, designated strains KMM 330^T and KMM 331, were isolated from a deep-sea sponge specimen and subjected to a polyphasic taxonomic study. The new isolates exhibited 16S rRNA gene sequence similarity of 99·9 %, and their mean level of DNA–DNA relatedness was 82 %. Phylogenetic analysis based on their 16S rRNA gene sequences placed the strains within the genus Pseudomonas as an independent deep clade. Strain KMM 330^T shared highest sequence similarity (96·3 %) with each of Pseudomonas fulva NRIC 0180^T, Pseudomonas parafulva AJ 2129^T and Pseudomonas luteola IAM 13000^T; sequence similarity to other recognized species of the genus Pseudomonas was below 95·7 %. The marine sponge isolates KMM 330^T and KMM 331 could be distinguished from the other recognized Pseudomonas species based on a unique combination of their phenotypic characteristics, including growth in 8 or 10 % NaCl, the absence of pigments, the inability to denitrify and lack of carbohydrate utilization. On the basis of phylogenetic analysis, physiological and biochemical characterization, strains KMM 330^T and KMM 331 should be classified as a novel species of the genus Pseudomonas, for which the name Pseudomonas pachastrellae sp. nov. is proposed. The type strain is KMM 330^T (=JCM 12285^T=NRIC 0583^T=CCUG 46540^T).

Published online ahead of print on 18 November 2004 as DOI 10.1099/ijs.0.63176-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of KMM 330^T and KMM 331 are AB125366 and AB125367, respectively.

Images of cell morphology, a thin-layer chromatogram of polar lipids and a maximum-likelihood tree are available as supplementary figures in IJSEM Online.

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