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Photobacterium rosenbergii sp. nov. and Enterovibrio coralii sp. nov., vibrios associated with coral bleaching

¹ Microbial Resources Division and Brazilian Collection of Environmental and Industrial Micro-organisms (CBMAI), CPQBA, CP 6171, UNICAMP, CEP 13081-970, São Paulo, Brazil
² Laboratory for Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium
³ BCCM^TM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium
⁴ University of Plymouth, School of Biological Sciences, Drake Circus, Plymouth PL4 8AA, UK
⁵ Australian Institute of Marine Science, PMB 3, Townsville MC, Queensland 4810, Australia

Correspondence
F. L. Thompson
fabiano.thompson{at}terra.com.br

Six new Vibrio-like isolates originating from different species of bleached and healthy corals around Magnetic Island (Australia) were investigated using a polyphasic approach. Phylogenetic analyses based on 16S rRNA, recA and rpoA gene sequences split the isolates in two new groups. Strains LMG 22223^T, LMG 22224, LMG 22225, LMG 22226 and LMG 22227 were phylogenetic neighbours of Photobacterium leiognathi LMG 4228^T (95·6 % 16S rRNA gene sequence similarity), whereas strain LMG 22228^T was related to Enterovibrio norvegicus LMG 19839^T (95·5 % 16S rRNA gene sequence similarity). The two new groups can be distinguished from closely related species on the basis of several phenotypic features, including fermentation of D-mannitol, melibiose and sucrose, and utilization of different compounds as carbon sources, arginine dihydrolase activity, nitrate reduction, resistance to the vibriostatic agent O/129 and the presence of fatty acids 15 : 0 iso and 17 : 0 iso. The names Photobacterium rosenbergii sp. nov. (type strain LMG 22223^T=CBMAI 622^T=CC1^T) and Enterovibrio coralii sp. nov. (type strain LMG 22228^T=CBMAI 623^T=CC17^T) are proposed to accommodate these new isolates. The G+C contents of the DNA of the two type strains are respectively 47·6 and 48·2 mol%.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, recA and rpoA gene sequences determined in this study are shown in Table 1.

Neighbour-joining trees showing relationships among Vibrio-like species on the basis of rpoA and recA gene sequence analysis are available as supplementary material in IJSEM Online.

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