Int J Syst Evol Microbiol 55 (2005), 907-911; DOI 10.1099/ijs.0.63488-0
© 2005 International Union of Microbiological Societies
Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile
Isao Yumoto1,2,
Kikue Hirota1,
Toshitaka Goto1,2,
Yoshinobu Nodasaka3 and
Kenji Nakajima1
1 Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
2 Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan
3 Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan
Correspondence
Isao Yumoto
i.yumoto{at}aist.go.jp
A halophilic and halotolerant, facultatively alkaliphilic strain, K11T, was isolated from soil obtained from Oshyamanbe, Oshima, Hokkaido, Japan. The isolate grew at pH 7–10. It was non-motile, Gram-positive and aerobic. Cells comprised straight rods and produced ellipsoidal spores. The isolate grew in 0–20 % NaCl, with optimum growth at 7 % NaCl, and hydrolysed casein, gelatin, starch, DNA and Tweens 20, 40, 60 and 80. The major isoprenoid quinone was menaquinone-7, and the cellular fatty acid profile consisted of significant amounts of C15 branched-chain acids, iso C15 : 0 and anteiso C15 : 0. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain K11T was a member of group 6 [Nielsen et al., FEMS Microbiol Lett 117 (1994), 61–66] (alkaliphiles) of the genus Bacillus. DNA–DNA hybridization revealed a low relatedness (14 %) of the isolate to its closest phylogenetic neighbour, Bacillus clausii. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic data and DNA–DNA relatedness data, it was concluded that K11T (=JCM 12663T=NCIMB 14023T) merits classification as the type strain of a novel species, for which the name Bacillus oshimensis sp. nov. is proposed.
Published online ahead of print on 18 November 2004 as DOI 10.1099/ijs.0.63488-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Bacillus oshimensis K11T is AB188090.
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Copyright © 2005 by the International Union of Microbiological Societies.
