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Methanofollis formosanus sp. nov., isolated from a fish pond

Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC

Correspondence
Mei-Chin Lai
mclai{at}dragon.nchu.edu.tw

A mesophilic, hydrogenotrophic methanogen, strain ML15^T, was isolated from an aquaculture fish pond near Wang-gong, Taiwan. The cells were irregular cocci, non-motile, 1·5–2·0 µm in diameter and Gram-negative. Cells of strain ML15^T lysed easily in the presence of SDS (0·1 g l^–1) and the S-layer protein had an M_r of 138 800. The catabolic substrates utilized by this strain included formate and H₂/CO₂, but not acetate, methanol, trimethylamine or secondary alcohols. Growth did not occur in minimal medium, but was observed when yeast extract and tryptone were added. Strain ML15^T grew fastest at 37 °C, pH 6·6–7·0 and with 3 % NaCl. Acetate was not required for cell growth. Trace amounts of tungstate promoted cell growth. The G+C contents of DNA of Methanofollis aquaemaris N2F9704^T and strain ML15^T were 59·1 and 58·4 mol%, respectively. Sequence analysis of the 16S rRNA genes of strain ML15^T and selected Methanofollis species revealed similarities of 95–97 %. Based on the data presented here, it is proposed that strain ML15^T (=OCM 789^T=DSM 15483^T) represents a novel species, Methanofollis formosanus sp. nov.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ML15^T is AY186542.

An EM of strain ML15^T and graphs showing the effects of temperature, pH and salinity on growth of strain ML15^T are available as supplementary material in IJSEM Online.

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