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Microbacterium oleivorans sp. nov. and Microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading Gram-positive bacteria

¹ Referat Geomikrobiologie, Bundesanstalt für Geowissenschaften und Rohstoffe, Stilleweg 2, D-30655 Hannover, Germany
² Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany

Correspondence
Axel Schippers
A.Schippers{at}bgr.de

A taxonomic study of two crude-oil-degrading, Gram-positive bacterial strains, designated BAS69^T and BNP48^T, revealed that they represent two novel Microbacterium species. 16S rRNA gene sequence similarity to their closest phylogenetic neighbours was 98·5 % for BAS69^T (Microbacterium paraoxydans DSM 15019^T and Microbacterium saperdae DSM 20169^T) and 99 % for BNP48^T (Microbacterium luteolum DSM 20143^T). Levels of DNA–DNA relatedness to the closest phylogenetic neighbours of both strains were between 11 and 38 %. According to phylogenetic analysis, the two strains are distinguishable from all recognized species of Microbacterium. Morphological and physiological characteristics of strains BAS69^T and BNP48^T were different from those of phylogenetically closely related Microbacterium species. The diamino acid in the cell-wall peptidoglycan of BAS69^T is lysine and of BNP48^T is ornithine. The major menaquinones are MK-11 and MK-12 for both strains. Based on their ability to degrade crude oil, the name Microbacterium oleivorans sp. nov. is proposed for strain BAS69^T (=DSM 16091^T=NCIMB 14003^T) and Microbacterium hydrocarbonoxydans is proposed for strain BNP48^T (=DSM 16089^T=NCIMB 14002^T).