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Thioreductor micantisoli gen. nov., sp. nov., a novel mesophilic, sulfur-reducing chemolithoautotroph within the -Proteobacteria isolated from hydrothermal sediments in the Mid-Okinawa Trough

¹ Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
² Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan

Correspondence
Satoshi Nakagawa
nakasato{at}kais.kyoto-u.ac.jp

A novel mesophilic, hydrogen-oxidizing, sulfur-reducing bacterium, designated strain BKB25Ts-Y^T, was isolated from hydrothermal sediments at Iheya North in the Mid-Okinawa Trough, Japan. Cells were Gram-negative, motile rods (1·8–2·1 µm long and 0·5–0·7 µm wide). The isolate was a strictly anaerobic chemolithoautotroph capable of using molecular hydrogen as the sole energy source and carbon dioxide as the sole carbon source. Elemental sulfur and nitrate served as electron acceptors, respectively yielding hydrogen sulfide and ammonium. Growth was observed at 20–42 °C (optimum 32 °C; 3 h doubling time), pH 5·0–6·5 (optimum 6·0) and in the presence of 2·0–4·0 % NaCl (optimum 2·5 %) via respiratory S⁰ reduction with H₂. The G+C content of the genomic DNA was 37·2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate represented the first strain for which taxonomic properties have been characterized within the previously uncultivated -Proteobacteria Group G. On the basis of the physiological and molecular properties of the novel isolate, the genus name Thioreductor gen. nov. is proposed, with Thioreductor micantisoli sp. nov. as the type species. The type strain is BKB25Ts-Y^T (=JCM 12457^T=DSM 16661^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BKB25Ts-Y^T is AB175498.

A figure showing the effects of temperature, pH and NaCl concentration on growth of strain BKB25Ts-Y^T is available as supplementary material in IJSEM Online.

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