HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) SEM and fatty acid composition Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mahendra, S. Articles by Alvarez-Cohen, L. Search for Related Content PubMed PubMed Citation Articles by Mahendra, S. Articles by Alvarez-Cohen, L. Agricola Articles by Mahendra, S. Articles by Alvarez-Cohen, L.

Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxane

Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720-1710, USA

Correspondence
Lisa Alvarez-Cohen
alvarez{at}ce.berkeley.edu

An actinomycete strain (CB1190^T) was previously isolated from industrial sludge contaminated with 1,4-dioxane. The cells of this culture are Gram-positive and exhibit branching aerial and vegetative mycelium. Analysis of the 16S rRNA gene sequence indicates that the strain belongs to the genus Pseudonocardia, closely related to Pseudonocardia hydrocarbonoxydans, P. sulfidoxydans and P. halophobica. Physiological and biochemical characteristics of CB1190^T are different from those of other known Pseudonocardia species. The novel organism described here is distinguished by its ability to grow on 1,4-dioxane, which is a probable human carcinogen. This culture can also grow on tetrahydrofuran, gasoline aromatics and several other toxic environmental contaminants. Strain CB1190^T is capable of fixing dinitrogen. The predominant fatty acids are 16 : 0 iso, 16 : 1 iso cis9 and 17 : 1 iso cis9. The major phospholipid fatty acids are 16 : 0 iso, 16 : 0 10-Me and 17 : 0 10-Me. The peptidoglycan belongs to type A1, meso-diaminopimelic acid. The major menaquinone is MK-8 (H₄). Mycolic acids are absent. The G+C content is 74 mol%. Based on morphological, physiological, chemotaxonomic and phylogenetic evidence, it is proposed that strain CB1190^T (=ATCC 55486^T=DSM 44775^T) be classified as the type strain of a novel species, Pseudonocardia dioxanivorans sp. nov. Further studies with this organism will provide insights into metabolic pathways, responsible enzymes, kinetics and the fate of 1,4-dioxane in the environment.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Pseudonocardia dioxanivorans CB1190^T is AY340622.

A scanning electron micrograph of cells of strain CB1190^T and details of fatty acid compositions are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				J. Schafer, H.-J. Busse, and P. Kampfer Pseudonocardia parietis sp. nov., from the indoor environment Int J Syst Evol Microbiol, October 1, 2009; 59(10): 2449 - 2452. [Abstract] [Full Text] [PDF]

				K. Skinner, L. Cuiffetti, and M. Hyman Metabolism and Cometabolism of Cyclic Ethers by a Filamentous Fungus, a Graphium sp. Appl. Envir. Microbiol., September 1, 2009; 75(17): 5514 - 5522. [Abstract] [Full Text] [PDF]

				K. Duangmal, A. Thamchaipenet, A. Matsumoto, and Y. Takahashi Pseudonocardia acaciae sp. nov., isolated from roots of Acacia auriculiformis A. Cunn. ex Benth. Int J Syst Evol Microbiol, June 1, 2009; 59(6): 1487 - 1491. [Abstract] [Full Text] [PDF]

				Q. Gu, H. Luo, W. Zheng, Z. Liu, and Y. Huang Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from surface-sterilized Oroxylum indicum root. Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2193 - 2197. [Abstract] [Full Text] [PDF]