Int J Syst Evol Microbiol 55 (2005), 589-592; DOI 10.1099/ijs.0.63205-0
© 2005 International Union of Microbiological Societies
Biochemical and genetic evidence for the transfer of Enterococcus solitarius Collins et al. 1989 to the genus Tetragenococcus as Tetragenococcus solitarius comb. nov.
Saïd Ennahar1,2 and
Yimin Cai1
1 National Institute of Livestock and Grassland Science, Department of Animal Feeding and Management, Nishinasuno, Tochigi 329-2793, Japan
2 Laboratoire de Chimie Analytique et Sciences de l'Aliment (UMR 7512), Faculté de Pharmacie, Université Louis Pasteur, 74, route du Rhin, 67400 Illkirch, France
Correspondence
Saïd Ennahar
ennahar{at}pharma.u-strasbg.fr
Phylogenetic analysis of 16S rRNA gene sequences revealed that Enterococcus solitarius is not a member of the genus Enterococcus, but is related to species of the genus Tetragenococcus. On a phylogenetic tree, E. solitarius clustered with Tetragenococcus halophilus and Tetragenococcus muriaticus, with which it showed the highest 16S rRNA gene sequence similarity level (about 94 %). Phenotypic studies indicated that E. solitarius was also unable to produce acid from lactose, providing further evidence of its affiliation to the genus Tetragenococcus. DNA hybridization studies indicated that E. solitarius was clearly a separate species, different from T. halophilus and T. muriaticus (reassociation levels of about 23 and 54 %, respectively). As suggested in previous studies, E. solitarius is closely related to but clearly distinct from T. halophilus. Based upon properties that taxonomically distinguish it from species of the genus Enterococcus, it is proposed that E. solitarius be transferred to the genus Tetragenococcus and reclassified as Tetragenococcus solitarius comb. nov. (type strain, 885/78T=ATCC 49428T=CCUG 29293T=CIP 103330T=DSM 5634T=JCM 8736T=LMG 12890T=NCTC 12193T).
Published online ahead of print on 24 September 2004 as DOI 10.1099/ijs.0.63205-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Tetragenococcus solitarius DSM 5634T is AJ301840.
Results of DNADNA hybridization experiments are available as supplementary material in IJSEM Online.
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Copyright © 2005 by the International Union of Microbiological Societies.