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Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process

¹ Department of Biological and Environmental Science, University of Jyväskylä, FIN-40014 Jyväskylä, Finland
² Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Vienna, Austria
³ Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Vienna, Austria
⁴ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
⁵ Finnish Forest Research Institute, Eteläranta 55, FIN-96300 Rovaniemi, Finland

Correspondence
Marja Tiirola
mtiirola{at}jyu.fi

A polychlorophenol-degrading strain, designated MT1^T, and three MT1-like strains, MT101, MT103 and MT104, were isolated from a cold (4–8 °C) fluidized-bed process treating chlorophenol-contaminated groundwater in southern Finland. The organisms were Gram-negative, rod-shaped, catalase-positive, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belonged to the -4 subclass of the Proteobacteria and were members of the genus Novosphingobium. The highest 16S rRNA gene sequence similarity observed for these strains was 96·5 % with the type strains of Novosphingobium hassiacum, Novosphingobium aromaticivorans and Novosphingobium subterraneum. Chemotaxonomic data (major ubiquinone: Q-10; major polyamine: spermidine; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; major fatty acids: 18 : 17c, 16 : 17c and 2-OH 14 : 0) as well as the ability to reduce nitrate supported the affiliation of the strains to the genus Novosphingobium. Based on the phylogenetic analysis, whole-cell fatty acid composition as well as biochemical and physiological characteristics, the MT1-like strains were highly similar and could be separated from all recognized Novosphingobium species. The novel species Novosphingobium lentum sp. nov. is proposed to accommodate strains MT1^T (=DSM 13663^T=CCUG 45847^T), MT101 (=CCUG 45849), MT103 (=CCUG 45850) and MT104 (=CCUG 45851).

Published online ahead of print on 8 October 2004 as DOI 10.1099/ijs.0.63386-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MT1^T is AJ303009.

Phylogenetic trees, a Euclidean distance dendrogram, a polar lipid profile and whole-cell fatty acid profiles of the strains studied are available as supplementary material in IJSEM Online.

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