HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vinuesa, P. Articles by Martínez-Romero, E. Search for Related Content PubMed PubMed Citation Articles by Vinuesa, P. Articles by Martínez-Romero, E. Agricola Articles by Vinuesa, P. Articles by Martínez-Romero, E.

Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta

¹ Centro de Ciencias Genómicas, UNAM, Av. Universidad s/n, Col. Chamilpa, CP 62210, AP. 565-A, Cuernavaca, Morelos, Mexico
² FB Biologie, FG für Zellbiologie und Angewandte Botanik, Philipps Universität Marburg, Karl von Frisch Str., D-35032 Marburg, Germany
³ Departamento de Microbiología, Facultad de Farmacia, Universidad de La Laguna, Avda Astrofísico Sánchez s/n, La Laguna, Spain
⁴ Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie. Universiteit Gent K.L., Ledeganckstraat 35, B-9000 Gent, Belgium

Correspondence
Pablo Vinuesa
vinuesa{at}ccg.unam.mx

Highly diverse Bradyrhizobium strains nodulate genistoid legumes (brooms) in the Canary Islands, Morocco, Spain and the Americas. Phylogenetic analyses of ITS, atpD, glnII and recA sequences revealed that these isolates represent at least four distinct evolutionary lineages within the genus, namely Bradyrhizobium japonicum and three unnamed genospecies. DNA–DNA hybridization experiments confirmed that one of the latter represents a new taxonomic species for which the name Bradyrhizobium canariense is proposed. B. canariense populations experience homologous recombination at housekeeping loci, but are sexually isolated from sympatric B. japonicum bv. genistearum strains in soils of the Canary Islands. B. canariense strains are highly acid-tolerant, nodulate diverse legumes in the tribes Genisteae and Loteae, but not Glycine species, whereas acid-sensitive B. japonicum soybean isolates such as USDA 6^T and USDA 110 do not nodulate genistoid legumes. Based on host-range experiments and phylogenetic analyses of symbiotic nifH and nodC sequences, the biovarieties genistearum and glycinearum for the genistoid legume and soybean isolates, respectively, were proposed. B. canariense bv. genistearum strains display an overlapped host range with B. japonicum bv. genistearum isolates, both sharing monophyletic nifH and nodC alleles, possibly due to the lateral transfer of a conjugative chromosomal symbiotic island across species. B. canariense is the sister species of B. japonicum, as inferred from a maximum-likelihood Bradyrhizobium species phylogeny estimated from congruent glnII+recA sequence partitions, which resolves eight species clades. In addition to the currently described species, this phylogeny uncovered the novel Bradyrhizobium genospecies alpha and beta and the photosynthetic strains as independent evolutionary lineages. The type strain for B. canariense is BTA-1^T (=ATCC BAA-1002^T=LMG 22265^T=CFNE 1008^T).

Published online ahead of print on 24 September 2004 as DOI 10.1099/ijs.0.63292-0.

The GenBank/EMBL/DDBJ accession number for the rrs sequence of strain BC-C2 is AY577427; those for the ITS1 sequences are AY386703–AY386705, AY386707, AY386708, AY386712–AY386718, AY386721, AY386722, AY386734, AY599094 and AY599095.

Sequence accession numbers for new Bradyrhizobium sequences used and generated in this study, the figures discussed in the text and our final and concluding remarks are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				F. Q. Wang, E. T. Wang, J. Liu, Q. Chen, X. H. Sui, W. F. Chen, and W. X. Chen Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1192 - 1199. [Abstract] [Full Text] [PDF]

				T. Stepkowski, C. E. Hughes, I. J. Law, L. Markiewicz, D. Gurda, A. Chlebicka, and L. Moulin Diversification of Lupine Bradyrhizobium Strains: Evidence from Nodulation Gene Trees Appl. Envir. Microbiol., May 15, 2007; 73(10): 3254 - 3264. [Abstract] [Full Text] [PDF]

				M. G. Germano, P. Menna, F. L. Mostasso, and M. Hungria RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species Int J Syst Evol Microbiol, January 1, 2006; 56(1): 217 - 229. [Abstract] [Full Text] [PDF]

				T. Stepkowski, L. Moulin, A. Krzyzanska, A. McInnes, I. J. Law, and J. Howieson European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa Appl. Envir. Microbiol., November 1, 2005; 71(11): 7041 - 7052. [Abstract] [Full Text] [PDF]