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Microbacterium halotolerans sp. nov., isolated from a saline soil in the west of China

Wen-Jun Li^1,2,, Hua-Hong Chen^1,3,, Chang-Jin Kim², Dong-Jin Park², Shu-Kun Tang¹, Jae-Chan Lee², Li-Hua Xu¹ and Cheng-Lin Jiang¹

¹ The Key Laboratory for Microbial Resources of the Ministry of Education, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China
² Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea
³ Department of Chemistry, Chuxiong Normal College, Chuxiong, Yunnan, 675000, People's Republic of China

Correspondence
Cheng-Lin Jiang
lihxu{at}ynu.edu.cn or
wjli{at}ynu.edu.cn

A Gram-positive short rod isolated from a saline soil in China was characterized using a polyphasic approach. This actinobacterium grew over a wide salinity range [0–15 % NaCl, 0–20 % KCl and 0–30 % MgCl₂.6H₂O (w/v); optimum concentrations for growth were 5 % NaCl, 5–10 % KCl, 10 % MgCl₂.6H₂O]. The optimum growth temperature and pH were 28–30 °C and 7·0–8·0, respectively. Chemotaxonomic features (peptidoglycan-type B2 with glycolyl residues; major menaquinones MK-10 and MK-11; predominating iso- and anteiso-branched cellular fatty acids; DNA G+C content 66·5 mol%) placed this organism within the genus Microbacterium. 16S rRNA gene sequence analysis confirmed this classification of the strain, but showed that it was distinct from its nearest neighbours. It formed a separate branch with type strains of the genus Microbacterium, and also shared low sequence similarity with them (<96 %). Based on phenotypic and molecular taxonomic results, it is proposed that the unknown isolate should be classified as a novel species in the genus Microbacterium, for which the name Microbacterium halotolerans sp. nov. is proposed. The type strain is YIM 70130^T (=KCTC 19017^T=CIP 108071^T).

Published online ahead of print on 9 July 2004 as DOI 10.1099/ijs.0.63239-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 70130^T is AY376165.

Detailed phenotypic characteristics and a phylogenetic dendrogram of strain YIM 70130^T and other Microbacterium species are available as supplementary material in IJSEM Online.

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