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Int J Syst Evol Microbiol 55 (2005), 479-486; DOI  10.1099/ijs.0.02852-0
© 2005 International Union of Microbiological Societies

Brevundimonas mediterranea sp. nov., a non-stalked species from the Mediterranean Sea

Ingo Fritz1, Carsten Strömpl2, Denis I. Nikitin3, A. M. Lysenko3 and Wolf-Rainer Abraham2

1 Max-Planck-Institute for Molecular Genetics, Department of Vertebrate Genomics, Ihnestraße 73, 14195 Berlin, Germany
2 GBF – National Research Center for Biotechnology, Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany
3 Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Octyabrya 7, korp 2, Moscow 117811, Russia

Correspondence
Wolf-Rainer Abraham
wab{at}gbf.de

Six strains of Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from the Mediterranean Sea. 16S rRNA gene sequence analysis indicated that the strains were affiliated within the alphaproteobacterial genus Brevundimonas, with Brevundimonas intermedia (99·4 %) and Brevundimonas vesicularis (99·2 %) as their closest relatives. This affiliation was supported by chemotaxonomic data (major polar lipids: phosphatidyl diacylglycerol, sulfoquinovosyl diacylglycerol and phosphatidyl glucopyranosyl diacylglycerol; major fatty acids: C18 : 1, C16 : 0, C16 : 1, C15 : 0, C17 : 1{omega}8c, 11-Me-C18 : 1{omega}5t). The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the strains from all recognized Brevundimonas species. The strains therefore represent a novel species, for which the name Brevundimonas mediterranea sp. nov. is proposed, with the type strain V4.BO.10T (=LMG 21911T=CIP 107934T).


Abbreviations: LMW-RNA, low-molecular-mass RNAs (tRNAs and rRNAs); OTU, operational taxonomic unit; PGL, phosphatidyl glucopyranosyl diacylglycerol; RAPD, randomly amplified polymorphic DNA

Published online ahead of print on 5 November 2004 as DOI 10.1099/ijs.0.02852-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains V4.BO.07, V4.BO.10T, LMG 9567t1, LMG 11070, LMG 9564, V4.BO.05, V4.BO.22, V4.BO.18, V4.BE.56, V4.BO.27, V4.BE.49 and V4.BP.05 are AJ227800, AJ227801, AJ244647–AJ244649 and AJ244704–AJ244710.

Strain details, transmission electron micrographs, protein profiles and RAPD profiles are available as supplementary material in IJSEM Online.




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