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Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov.

¹ Department of Clinical Microbiology, Aarhus University Hospital, DK-8000 Aarhus, Denmark
² Department of Medical Microbiology and Immunology, Aarhus University, DK-8000 Aarhus, Denmark
³ Department of Clinical Microbiology, Hillerød Hospital, DK-3400 Hillerød, Denmark

Correspondence
Niels Nørskov-Lauritsen
norskov{at}microbiology.au.dk

The phylogeny of human isolates of Haemophilus species was estimated based on partial sequences of four separate housekeeping genes. The clustering of each set of sequences was in accordance with speciation of the strains with few exceptions: of 108 gene fragments examined, only three appeared to have been subject to recombination events across the species barrier. Housekeeping gene similarity supported previous DNA–DNA hybridization data for the genus rather than the phylogeny inferred from 16S rRNA gene sequence comparison. The similarity of sequences of Haemophilus parainfluenzae with those of Haemophilus influenzae suggested preservation of the former species in the genus Haemophilus. Three strains representing a novel taxon were unique with respect to the four investigated gene loci. 16S rRNA gene sequence analysis suggested that this taxon belonged to the Parainfluenzae cluster. DNA–DNA hybridization data supported this generic placement. Nine strains of the novel taxon were available for analysis. They were distinct from representatives of all current species of the genus Haemophilus by conventional phenotypic characterization. Genotypic and phenotypic data show that the strains merit recognition as a novel species of Haemophilus. The name Haemophilus pittmaniae sp. nov. is proposed, with HK 85^T (=CCUG 48703^T=NCTC 13334^T) as the type strain.

Published online ahead of print on 24 September 2004 as DOI 10.1099/ijs.0.63325-0.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are AJ289631, AJ289636, AJ289639, AJ289640, AJ289644, AJ289650, AJ289653, AJ289659, AJ289664–AJ289671, AJ289675, AJ289677, AJ289679–AJ289681, AJ289685, AJ289688, AJ289690, AJ289694, AJ290754, AJ290755, AJ290758, AJ295746, AJ438112, AJ585268–AJ585341, AY597040 and AY736136–AY736145.

A table showing the origins of human isolates of Haemophilus pittmaniae sp. nov. is available as supplementary material in IJSEM Online.

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