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Bacillus patagoniensis sp. nov., a novel alkalitolerant bacterium from the rhizosphere of Atriplex lampa in Patagonia, Argentina

Nelda Olivera^1,, Faustino Siñeriz^1,2 and Javier D. Breccia^1,3

¹ PROIMI Planta Piloto de Procesos Industriales Microbiológicos, Av. Belgrano y Pasaje Caseros, 4000 San Miguel de Tucumán, Tucumán, Argentina
² Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina
³ Cátedra de Microbiología de Alimentos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Uruguay 151, 6300 Santa Rosa, La Pampa, Argentina

Correspondence
Nelda Olivera
olivera{at}cenpat.edu.ar

A Gram-positive, rod-shaped, spore-forming bacterium (PAT 05^T) was isolated from the rhizosphere of the perennial shrub Atriplex lampa in north-eastern Patagonia, Argentina. Its overall biochemical and physiological characteristics indicated that this strain should be placed in the alkaliphilic Bacillus group. Strain PAT 05^T grew at pH 7–10 (optimum pH 8), but not at pH 6. Its DNA G+C content was 39·7 mol%. Sequence analysis of the 16S rRNA gene of PAT 05^T revealed the closest match (99·6 % similarity) with Bacillus sp. DSM 8714. The highest level of DNA–DNA relatedness (88·6 %) was also found with this strain. On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, G+C content and DNA–DNA hybridization data, strain PAT 05^T is related at the species level to Bacillus sp. DSM 8714, a member of a group referred as phenon 4a by Nielsen et al. [Nielsen, P., Fritze, D. & Priest, F. G. (1995). Microbiology 141, 1745–1761], which still lacks taxonomic standing. These results support the proposal of strain PAT 05^T (=DSM 16117^T=ATCC BAA-965^T) as the type strain of Bacillus patagoniensis sp. nov.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PAT 05^T is AY258614.

An electron photomicrograph of a negatively stained cell of strain PAT 05^T is available as supplementary material in IJSEM Online.

Present address: Centro Nacional Patagónico, Blvd Brown s/n, 9120 Puerto Madryn, Chubut, Argentina.

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