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Nocardia takedensis sp. nov., isolated from moat sediment and scumming activated sludge

¹ Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan
² NITE Biological Resource Center, Department of Biotechnology, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu 292-0818, Japan
³ Department of Civil and Environmental Engineering, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan
⁴ Water Quality Control Laboratory, Nihon Hels Industry Corporation, Higashigoken-cho 3-25, Shinjuku-ku, Tokyo 162-0813, Japan

Correspondence
Masayuki Hayakawa
hayakawa{at}ab11.yamanashi.ac.jp

Chemotaxonomic and morphological characterization of two actinomycete strains, MS1-3^T and AS4-2, respectively isolated from moat sediment and scumming activated sludge, was carried out. This characterization clearly demonstrated that strains MS1-3^T and AS4-2 belong to the genus Nocardia. 16S rRNA gene sequencing studies showed that these isolates are most closely related to Nocardia beijingensis (98·1–98·3 % similarity), Nocardia brasiliensis (97·9–98·0 %) and Nocardia tenerifensis (97·8–97·9 %). However, the results of DNA–DNA hybridizations and physiological and biochemical tests showed that strains MS1-3^T and AS4-2 could be differentiated from their closest phylogenetic relatives both genotypically and phenotypically. It is proposed that the two isolates be classified as representatives of a novel species of Nocardia, Nocardia takedensis sp. nov. The type strain is MS1-3^T (=NBRC 100417^T=DSM 44801^T); AS4-2 (=NBRC 100418=DSM 44802) is a reference strain.

Published online ahead of print on 10 September 2004 as DOI 10.1099/ijs.0.63189-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Nocardia takedensis MS1-3^T and AS4-2 are AB158277 and AB158278.

SEMs of cells of strain MS1-3^T and an extended phylogenetic tree derived from 16S rRNA gene sequences are available as supplementary material in IJSEM Online.

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