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Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough

Laurent Toffin^1,, Klaus Zink², Chiaki Kato³, Patricia Pignet⁴, Adeline Bidault¹, Nadège Bienvenu¹, Jean-Louis Birrien¹ and Daniel Prieur¹

¹ UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
² GeoForschungsZentrumPotsdam (GFZ), Telegrafenberg, D-14473 Potsdam, Germany
³ Extremobiosphere Research Centre, Japan Agency for Marine–Earth Science and Technology (JAMSTEC), 2–15 Natsushima-cho, Yokosuka 237-0061, Japan
⁴ Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, Département de Valorisation des Produits, IFREMER, Centre de Brest, BP 70, F-29280 Plouzané, France

Correspondence
Laurent Toffin
toffin{at}jamstec.go.jp

A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20^T) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaCl concentration range for growth was 0–120 g l^–1, with the optimum at 10–20 g l^–1. The temperature range for growth at pH 7·0 was 4–50 °C, with the optimum at 37–40 °C. The optimum pH for growth was 7·0–8·0. The optimum pressure for growth was 0·1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %); phosphatidylethanolamines (9 %) were minor components. The fatty acid composition was dominated by side chains of 16 : 0, 14 : 0 and 16 : 1. The G+C content of the genomic DNA was 42 mol%. On the basis of 16S rRNA gene sequence analysis and the secondary structure of the V6 region, this organism was found to belong to the genus Marinilactibacillus and was closely related to Marinilactibacillus psychrotolerans M13-2^T (99 %), Marinilactibacillus sp. strain MJYP.25.24 (99 %) and Alkalibacterium olivapovliticus strain ww2-SN4C (97 %). Despite the high similarity between their 16S rRNA gene sequences (99 %), the DNA–DNA hybridization levels were less than 20 %. On the basis of physiological and genetic characteristics, it is proposed that this organism be classified as a novel species, Marinilactibacillus piezotolerans sp. nov. The type strain is LT20^T (=DSM 16108^T=JCM 12337^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Marinilactibacillus piezotolerans LT20^T is AY485792.

Details of the morphology, differential phenotypic characteristics and the effect of pH on glucose fermentation in strain LT20^T are available as supplementary material in IJSEM Online.

Present address: Extremobiosphere Research Centre, JAMSTEC, 2–15 Natsushima-cho, Yokosuka 237-0061, Japan.

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