HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ma, K. Articles by Dong, X. Search for Related Content PubMed PubMed Citation Articles by Ma, K. Articles by Dong, X. Agricola Articles by Ma, K. Articles by Dong, X.

Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

Two methanogenic strains, 8-2^T and 4-1, with rod-shaped (0·4–0·5x3–5 µm), non-motile cells, sometimes observed in chains, were isolated from two anaerobic digesters in Beijing, China. The two strains used H₂/CO₂ and formate for growth and produced methane. The temperature range for growth was 25–50 °C, with fastest growth at 37 °C. The pH ranges for growth and methane production were 6·5–8·0 for strain 8-2^T and 6·8–8·6 for strain 4-1, with the fastest growth at pH 7·2 for strain 8-2^T and pH 7·5–7·7 for strain 4-1. The G+C content of genomic DNA for strain 8-2^T was 38·9 mol%. The similarity levels of the 16S rRNA sequence of strain 8-2^T with other species of the genus Methanobacterium ranged from 93·8 to 96·0 %. Based on the phylogenetic analysis and phenotypic characteristics, the novel species Methanobacterium beijingense sp. nov. is proposed, with the type strain 8-2^T (=DSM 15999^T=CGMCC 1.5011^T).

Published online ahead of print on 27 August 2004 as DOI 10.1099/ijs.0.63254-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 8-2^T and 4-1 are AY350742 and AY552778.

This article has been cited by other articles:

				H. Cadillo-Quiroz, E. Yashiro, J. B. Yavitt, and S. H. Zinder Characterization of the Archaeal Community in a Minerotrophic Fen and Terminal Restriction Fragment Length Polymorphism-Directed Isolation of a Novel Hydrogenotrophic Methanogen Appl. Envir. Microbiol., April 1, 2008; 74(7): 2059 - 2068. [Abstract] [Full Text] [PDF]

				K. Ma, X. Liu, and X. Dong Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor Int J Syst Evol Microbiol, January 1, 2006; 56(1): 127 - 131. [Abstract] [Full Text] [PDF]