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Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain

Correspondence
Antonio Ventosa
ventosa{at}us.es

Members of the genus Methylobacterium are ubiquitous in nature and can be isolated from almost any freshwater environment where dissolved oxygen exists. This genus is composed of a variety of pink-pigmented, facultatively methylotrophic (PPFM) bacteria. During a screening programme to monitor the bacterial population present in the drinking water of a municipal water supply in Seville (Spain) during the year 2003, five strains of PPFM bacteria were isolated and characterized. Analysis of their complete 16S rRNA gene sequences revealed that they constituted two separate phylogenetic groups (strains GP34^T and GR18, and strains GR16^T, GP22 and GP32, respectively) showing highest similarity to members of the genus Methylobacterium. The highest 16S rRNA sequence similarities of strain GP34^T were found with respect to the type strains of Methylobacterium radiotolerans (96·6 %) and Methylobacterium fujisawaense (96·4 %) and the highest 16S rRNA sequence similarities of strain GR16^T were to the type strains of Methylobacterium extorquens (96·0 %) and Methylobacterium rhodesianum (95·8 %). The G+C content of their DNA ranged from 66·5 to 67·8 mol%. DNA–DNA hybridization studies confirmed that they constituted two separate genospecies. On the basis of this phenotypic, phylogenetic and genotypic study, two novel species of the genus Methylobacterium are proposed: Methylobacterium hispanicum sp. nov., with type strain GP34^T (CECT 5997^T=CCM 7219^T=DSM 16372^T=CIP 108332^T), and Methylobacterium aquaticum sp. nov., with type strain GR16^T (CECT 5998^T=CCM 7218^T=DSM 16371^T=CIP 108333^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains GP34^T, GR18, GR16^T, GP22 and GP32 are AJ635304, AJ785570, AJ635303, AJ785571 and AJ785572, respectively.

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