Int J Syst Evol Microbiol 55 (2005), 183-189; DOI 10.1099/ijs.0.63330-0
© 2005 International Union of Microbiological Societies
Lebetimonas acidiphila gen. nov., sp. nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal fumarole in the Mariana Arc
Ken Takai1,
Hisako Hirayama1,
Tatsunori Nakagawa1,
Yohey Suzuki1,
Kenneth H. Nealson1,2 and
Koki Horikoshi1
1 Subground Animalcule Retrieval (SUGAR) Project, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
2 Department of Earth Sciences, University of Southern California, 3651 Trousdale Pkwy, Los Angeles, CA 90089-0740, USA
Correspondence
Ken Takai
kent{at}jamstec.go.jp
A novel thermophilic, acidophilic bacterium, designated strain Pd55T, was isolated from a self-temperature-recording in situ colonization system deployed in a hydrothermal diffusing flow (maximum temperature of 78 °C) at the TOTO caldera in the Mariana Arc. Cells of strain Pd55T were motile, short rods with a single polar flagellum. Growth was observed between 30 and 68 °C (optimum growth at 50 °C; 120 min doubling time) and between (initial) pH 4·2 and 7·0 (optimum at pH 5·2). The isolate was a strictly anaerobic chemolithoautotroph capable of using molecular hydrogen as sole energy source and carbon dioxide as sole carbon source. Elemental sulfur served as the sole electron acceptor to support growth. The G+C content of the genomic DNA was 34·0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was related to members of the genera Nautilia and Caminibacter, although it appeared to be a novel lineage prior to the divergence between Nautilia and Caminibacter. Strain Pd55T could also be differentiated from Nautilia and Caminibacter species on the basis of its physiological properties. It is, therefore, proposed that strain Pd55T (=JCM 12420T=DSM 16356T) represents the type strain of a novel species in a new genus, Lebetimonas acidiphila gen. nov., sp. nov.
Abbreviations: EPR, East Pacific Rise; MAR, Mid-Atlantic Ridge; STR-ISCS, self-temperature-recording in situ colonization system
Published online ahead of print on 6 August 2004 as DOI 10.1099/ijs.0.63330-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Pd55T is AB167820.
An electron micrograph of strain Pd55T (Fig. A) and graphs showing the effects of temperature, pH and NaCl concentration on the growth rate of strain Pd55T (Fig. Ba–c) are available as supplementary material in IJSEM Online.
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