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1 Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
2 National Institute of Livestock and Grassland Science, Nishinasuno, Tochigi 329-2793, Japan
3 Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Correspondence
Feng-Yan Bai
baify{at}sun.im.ac.cn
In an investigation of the yeast biota involved in silage deterioration, a considerable number of strains belonging to Saccharomyces and related genera were isolated from aerobically deteriorating corn silage in Tochigi, Japan. Analysis of sequences of the internal transcribed spacer and the large-subunit rRNA gene D1/D2 domain and electrophoretic karyotyping indicated that two of the strains, NS 14T and NS 26, represent a novel species with close phylogenetic relationships to Kazachstania servazzii and Kazachstania unispora. It is proposed that the novel species be named Kazachstania aerobia sp. nov., with NS 14T (=AS 2.2384T=CBS 9918T) as the type strain.
Published online ahead of print on 28 June 2004 as DOI 10.1099/ijs.0.63257-0.
The GenBank/EMBL/DDBJ accession numbers for the ITS region and 26S rRNA gene D1/D2 domain sequences determined in this study are AY582126AY582128.
A phylogenetic tree drawn from neighbour-joining analysis of the 26S rRNA gene D1/D2 domain sequences, depicting the relationships between Kazachstania aerobia sp. nov. and related taxa, is available as supplementary material in IJSEM Online.
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