IJSEM Faster Access from Outside North America
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary material
Right arrow Erratum
Right arrow Erratum (v55,p545)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fernández, E.
Right arrow Articles by Fernández-Garayzábal, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fernández, E.
Right arrow Articles by Fernández-Garayzábal, J. F.
Agricola
Right arrow Articles by Fernández, E.
Right arrow Articles by Fernández-Garayzábal, J. F.
Int J Syst Evol Microbiol 54 (2004), 2291-2296; DOI  10.1099/ijs.0.63145-0
© 2004 International Union of Microbiological Societies

Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants

Elena Fernández1, Verena Blume1, Patricia Garrido1, Matthew D. Collins2, Ana Mateos1, Lucas Domínguez1 and José F. Fernández-Garayzábal1

1 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avenida Puerta de Hierro s/n, Madrid 28040, Spain
2 School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK

Correspondence
José F. Fernández-Garayzábal
garayzab{at}vet.ucm.es

Six isolates of an unknown Gram-positive, catalase-negative, chain-forming, coccus-shaped organism isolated from ovine and caprine mastitis were characterized by phenotypic and molecular taxonomic methods. On the basis of cellular morphology and the results of biochemical tests, the organism was tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed that the organism is a member of the genus Streptococcus, with Streptococcus equi as its closest phylogenetic relative (98·8 % similarity). DNA–DNA pairing studies showed that the unidentified organism displayed more than 70 % relatedness to the type strains of S. equi subsp. equi and subsp. zooepidemicus. Despite the relatively high DNA–DNA reassociation values, biotyping and ribotyping allowed clear differentiation of the unknown bacterium from the two recognized subspecies of S. equi. On the basis of phenotypic and molecular genetic evidence, it is proposed that the unknown Streptococcus isolates from ovine and caprine mastitis be classified as a novel subspecies, Streptococcus equi subsp. ruminatorum subsp. nov. The type strain is CECT 5772T (=CCUG 47520T=Mt 167T).

Abbreviations: CAMP, Christie–Atkins–Munch-Petersen

Published online ahead of print on 28 June 2004 as DOI 10.1099/ijs.0.63145-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Streptococcus equi subsp. ruminatorum subsp. nov. strain CECT 5772T is AJ605748.

A full version of the dendrogram in Fig. 1, including a wider sample of Streptococcus species, is available as supplementary material in IJSEM Online.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
J. Lannergard, M. Flock, S. Johansson, J.-I. Flock, and B. Guss
Studies of Fibronectin-Binding Proteins of Streptococcus equi
Infect. Immun., November 1, 2005; 73(11): 7243 - 7251.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2004 by the International Union of Microbiological Societies.