| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR 1063, IRD/CIRAD/INRA/Agro-M/UMII, TA 10/J, Campus International de Baillarguet, 34398 Montpellier cedex 5, France
2 Laboratory of Microbiology, University of Gent, Ledeganckstraat 35, 9000 Gent, Belgium
Correspondence
Philippe Jourand
jourand{at}mpl.ird.fr
Data on 72 non-pigmented bacterial strains that specifically induce nitrogen-fixing root nodules on the legume species Crotalaria glaucoides, Crotalaria perrottetii and Crotalaria podocarpa are reviewed. By SDS-PAGE analysis of total protein patterns and by 16S rRNA PCR-RFLP, these strains form a homogeneous group that is separate from other legume root-nodule-forming bacteria. The 16S rRNA gene-based phylogeny indicates that these bacteria belong to the genus Methylobacterium. They can grow on C1 compounds such as methanol, formate and formaldehyde but not methylamine as sole carbon source, and carry an mxaF gene, encoding methanol dehydrogenase, which supports their methylotrophic metabolism. Presence of a nodA nodulation gene, and ability to nodulate plants of Crotalaria species and to fix nitrogen are features that separate the strains currently included in this group from other members of the genus Methylobacterium. The present study includes additional genotypic and phenotypic characterization of this novel Methylobacterium species, i.e. nifH gene sequence, morphology, physiology, enzymic and carbon source assimilation tests and antibiotic resistance. The name Methylobacterium nodulans sp. nov. (type strain, ORS 2060T=CNCM I 2342T=LMG 21967T) is proposed for this group of root-nodule-forming bacteria.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and partial nifH gene sequences of strain ORS 2060T are AF220763 and AJ512205, respectively.
This article has been cited by other articles:
|
|
 |
|
  M. Madhaiyan, S. Poonguzhali, S.-W. Kwon, and T.-M. Sa Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice Int J Syst Evol Microbiol, January 1, 2009; 59(1): 22 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Knief, L. Frances, F. Cantet, and J. A. Vorholt Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis Appl. Envir. Microbiol., April 1, 2008; 74(7): 2218 - 2228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Kang, J. Kim, H.-D. Shin, Y.-D. Nam, J.-W. Bae, C. O. Jeon, and W. Park Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2849 - 2853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, F. Sahr, T. Xue, and B. Sun Methylobacterium salsuginis sp. nov., isolated from seawater Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1699 - 1703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Aslam, C. S. Lee, K.-H. Kim, W.-T. Im, L. N. Ten, and S.-T. Lee Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood Int J Syst Evol Microbiol, March 1, 2007; 57(3): 566 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Madhaiyan, B.-Y. Kim, S. Poonguzhali, S.-W. Kwon, M.-H. Song, J.-H. Ryu, S.-J. Go, B.-S. Koo, and T.-M. Sa Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice Int J Syst Evol Microbiol, February 1, 2007; 57(2): 326 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Abanda-Nkpwatt, M. Musch, J. Tschiersch, M. Boettner, and W. Schwab Molecular interaction between Methylobacterium extorquens and seedlings: growth promotion, methanol consumption, and localization of the methanol emission site J. Exp. Bot., December 1, 2006; 57(15): 4025 - 4032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gallego, M. T. Garcia, and A. Ventosa Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water Int J Syst Evol Microbiol, February 1, 2006; 56(2): 339 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gallego, M. T. Garcia, and A. Ventosa Methylobacterium isbiliense sp. nov., isolated from the drinking water system of Sevilla, Spain Int J Syst Evol Microbiol, November 1, 2005; 55(6): 2333 - 2337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sy, A. C. J. Timmers, C. Knief, and J. A. Vorholt Methylotrophic Metabolism Is Advantageous for Methylobacterium extorquens during Colonization of Medicago truncatula under Competitive Conditions Appl. Envir. Microbiol., November 1, 2005; 71(11): 7245 - 7252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gallego, M. T. Garcia, and A. Ventosa Methylobacterium variabile sp. nov., a methylotrophic bacterium isolated from an aquatic environment Int J Syst Evol Microbiol, July 1, 2005; 55(4): 1429 - 1433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Trujillo, A. Willems, A. Abril, A.-M. Planchuelo, R. Rivas, D. Ludena, P. F. Mateos, E. Martinez-Molina, and E. Velazquez Nodulation of Lupinus albus by Strains of Ochrobactrum lupini sp. nov. Appl. Envir. Microbiol., March 1, 2005; 71(3): 1318 - 1327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gallego, M. T. Garcia, and A. Ventosa Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water Int J Syst Evol Microbiol, January 1, 2005; 55(1): 281 - 287. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
