| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Avenida Michoacán y la Purísima s/n, Col. Vicentina, 09340 México DF, Mexico
2 Institut de Recherche pour le Développement (IRD), Cicerón 609, Col. Los Morales, 11530 México DF, Mexico
3 Laboratoire de Microbiologie IRD, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille cedex 9, France
4 Central Research Laboratories, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-ku, Kawasaki-shi, 210-8681, Japan
5 Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Wasserturmstr. 3, 91054 Erlangen, Germany
6 LAMIB-CRSBAN, Département de Biochimie-Microbiologie, Unité de Formation et de Recherches en Sciences de la Vie et de la Terre, Université de Ouagadougou, 03 BP 7021, Ouagadougou 03, Burkina Faso
7 Leukaemia Foundation Research Unit, Queensland Institute of Medical Research, 300 Herston Rd, Herston QLD-4000, Australia
Correspondence
Hervé Macarie
herve.macarie{at}esil.univ-mrs.fr
Three mesophilic bacteria (strains AMX 26BT, UR374_02 and 12-3T) isolated respectively from an anaerobic digester, human urine and urban riverside soil were characterized. Cells were Gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum and had a strictly respiratory metabolism with O2 as the preferential terminal electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all strains clustered within the Xanthomonadaceae branch of the Proteobacteria. Isolates AMX 26BT and UR374_02 exhibited 100 % 16S rRNA gene sequence similarity and both were related to strain 12-3T (99·6 % similarity). The closest relative of all the isolates was Pseudoxanthomonas broegbernensis DSM 12573T (similarity 97·1–97·5 %), and they were equidistantly related to Xanthomonas species (95·4–96·6 %), Stenotrophomonas species (95·3–96·1 %) and Pseudoxanthomonas taiwanensis ATCC BAA-4040T (95·3–95·4 %). Chemotaxonomic and biochemical data (branched-chain cellular fatty acid pattern without C13 : 0 iso 3-OH, ubiquinone with eight isoprenoid units, limited range of substrates used, ability to reduce nitrite but not nitrate with the production of N2O) supported their affiliation to the genus Pseudoxanthomonas. The results of DNA–DNA hybridization and/or phenotypic analysis allowed them to be differentiated from the two Pseudoxanthomonas species with validly published names and showed that strain 12-3T was genomically and phenotypically distinct from the other two isolates. On the basis of these results, two novel species of the genus Pseudoxanthomonas are proposed: Pseudoxanthomonas mexicana sp. nov., consisting of strains AMX 26BT (=ATCC 700993T=CIP 106674T=JCM 11524T) (type strain) and UR374_02 (=DSM 15133), and Pseudoxanthomonas japonensis sp. nov., consisting of strain 12-3T (=CCUG 48231T=CIP 107388T=JCM 11525T). The report of these two novel species leads to the emendation of the description of the genus Pseudoxanthomonas and the re-evaluation of the phenotype of P. broegbernensis DSM 12573T necessitates the emendation of its description.
Published online ahead of print on 15 June 2004 as DOI 10.1099/ijs.0.02810-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains AMX 26BT, UR374_02 and 12-3T are respectively AF273082, AY124375 and AB008507.
Transmission electron micrographs, graphs showing anoxic growth in the presence of nitrite and a dendrogram comparing CFA profiles are available as supplementary material in IJSEM Online.
This article has been cited by other articles:
|
|
 |
|
  D. S. Lee, S. H. Ryu, H. W. Hwang, Y.-J. Kim, M. Park, J. R. Lee, S.-S. Lee, and C. O. Jeon Pseudoxanthomonas sacheonensis sp. nov., isolated from BTEX-contaminated soil in Korea, transfer of Stenotrophomonas dokdonensis Yoon et al. 2006 to the genus Pseudoxanthomonas as Pseudoxanthomonas dokdonensis comb. nov. and emended description of the genus Pseudoxanthomonas Int J Syst Evol Microbiol, September 1, 2008; 58(9): 2235 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Young, M.-J. Ho, A. B. Arun, W.-M. Chen, W.-A. Lai, F.-T. Shen, P. D. Rekha, and A. F. Yassin Pseudoxanthomonas spadix sp. nov., isolated from oil-contaminated soil Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1823 - 1827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Yoo, H.-Y. Weon, B.-Y. Kim, J.-H. Kim, Y.-K. Baek, S.-W. Kwon, S.-J. Go, and E. Stackebrandt Pseudoxanthomonas yeongjuensis sp. nov., isolated from soil cultivated with Korean ginseng Int J Syst Evol Microbiol, March 1, 2007; 57(3): 646 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Harada, S. Campbell, and Q. X. Li Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean. Int J Syst Evol Microbiol, May 1, 2006; 56(Pt 5): 1103 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Weon, B.-Y. Kim, J.-S. Kim, S.-Y. Lee, Y.-H. Cho, S.-J. Go, S.-B. Hong, W.-T. Im, and S.-W. Kwon Pseudoxanthomonas suwonensis sp. nov., isolated from cotton waste composts. Int J Syst Evol Microbiol, March 1, 2006; 56(Pt 3): 659 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-C. Yang, W.-T. Im, M. K. Kim, and S.-T. Lee Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov. Int J Syst Evol Microbiol, March 1, 2005; 55(2): 787 - 791. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
