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Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwiska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994

Pavel vec¹, Marc Vancanneyt², Ivo Sedláek¹, Katrien Engelbeen², Vlastimil ttina¹, Jean Swings² and Petr Petrá³

¹ Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic
² BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
³ Reference Laboratory for Staphylococci, National Institute of Public Health, robárova 48, 100 42, Praha 10, Czech Republic

Correspondence
Pavel vec
mpavel{at}sci.muni.cz

A polyphasic taxonomic approach was applied to strains of the species Staphylococcus vitulinus and Staphylococcus pulvereri in order to clarify their taxonomic relatedness. Four reference strains, representing both species, and seven strains isolated from human clinical material were characterized by biotyping, ribotyping and SDS-PAGE analysis of whole-cell proteins, and none of the screening approaches allowed the two taxa to be distinguished. DNA–DNA hybridization experiments between four selected representative strains, including the type strains, confirmed that Staphylococcus pulvereri is a later synonym of Staphylococcus vitulinus.

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