HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Li, X. Articles by Zhang, L. Search for Related Content PubMed PubMed Citation Articles by Li, X. Articles by Zhang, L. Agricola Articles by Li, X. Articles by Zhang, L.

Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium

¹ Department of Bio and Food Engineering, Dalian College of Light Industry, Dalian 116034, PR China
² IMA, National University of Singapore, Singapore 117612
³ Department of Botany, Hong Kong University, Hong Kong, PR China

Correspondence
Xianzhen Li
xianzhen{at}mail.com

Cells of strain LX3^T, isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 °C, unable to produce gas from lactose at 45 °C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56·4 mol%. Furthermore, comparison of 16S rRNA and rpoB gene sequences showed that the isolate clearly belongs to the genus Klebsiella. The closest phylogenetic relative was Klebsiella pneumoniae, there being 99·3 and 97·5 % similarity in 16S rRNA and rpoB gene sequences, respectively. DNA–DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus Klebsiella, indicating that the isolated strain and other species in the genus Klebsiella were not related at the species level. The isolate could be differentiated from other previously described members of the genus Klebsiella on the basis of phenotypic differences and 16S rRNA and rpoB gene sequence divergence, together with DNA–DNA reassociation data. Therefore, it is proposed that strain LX3^T (=DSM 16265^T=JCM 12419^T) should be classified as the type strain of a novel species of genus Klebsiella, Klebsiella singaporensis sp. nov.

This article has been cited by other articles:

				V. Merhej, T. Adekambi, I. Pagnier, D. Raoult, and M. Drancourt Yersinia massiliensis sp. nov., isolated from fresh water Int J Syst Evol Microbiol, April 1, 2008; 58(4): 779 - 784. [Abstract] [Full Text] [PDF]

				R. Stephan, S. Van Trappen, I. Cleenwerck, C. Iversen, H. Joosten, P. De Vos, and A. Lehner Enterobacter pulveris sp. nov., isolated from fruit powder, infant formula and an infant formula production environment Int J Syst Evol Microbiol, January 1, 2008; 58(1): 237 - 241. [Abstract] [Full Text] [PDF]

				R. Stephan, S. Van Trappen, I. Cleenwerck, M. Vancanneyt, P. De Vos, and A. Lehner Enterobacter turicensis sp. nov. and Enterobacter helveticus sp. nov., isolated from fruit powder Int J Syst Evol Microbiol, April 1, 2007; 57(4): 820 - 826. [Abstract] [Full Text] [PDF]

				M. S. Alves, R. C. d. S. Dias, A. C. D. de Castro, L. W. Riley, and B. M. Moreira Identification of Clinical Isolates of Indole-Positive and Indole-Negative Klebsiella spp. J. Clin. Microbiol., October 1, 2006; 44(10): 3640 - 3646. [Abstract] [Full Text] [PDF]