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Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids

¹ Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
² Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Minaminosawa, Minami-ku, Sapporo 005-8601, Japan
³ Laboratory of Electron Microscopy, School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan
⁴ Asahi Vet Japan Co., Ltd, 724-1 Midorimachi, Shiraoi-cho, Hokkaido 059-0931, Japan

Correspondence
Isao Yumoto
i.yumoto{at}aist.go.jp

In a screening campaign to isolate strains with the ability to remove the bad smell associated with animal faeces, strain MA001^T was isolated from a soil sample obtained from Shizuoka prefecture, Japan. The isolate grew at pH 6–9 but not at pH 10. Cells were Gram-positive, straight rods with peritrichous flagella and produced ellipsoidal spores. The isolate was positive for catalase and oxidase tests but negative for indole production, deamination of phenylalanine and H₂S production. The isolate did not produce acid from any carbohydrates tested and could not grow in more than 2 % NaCl. The DNA G+C content was 39·4 mol%. The cellular fatty acids profile consisted of significant amount of C₁₅ branched-chain fatty acids, iso-C_{15 : 0} and anteiso-C_{15 : 0}. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain MA001^T was closely related to Bacillus simplex and Bacillus psychrosaccharolyticus. DNA–DNA hybridization revealed a low relatedness of the isolate to several phylogenetically close neighbours (less than 9 %). On the basis of the phenotypic characteristics observed, phylogenetic data based on 16S rRNA gene sequencing and DNA–DNA relatedness data, it is concluded that the isolate should be classified as representing a novel species, for which the name Bacillus asahii is proposed. The type strain is MA001^T (=JCM 12112^T=NCIMB 13969^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MA001^T is AB109209.

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