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Int J Syst Evol Microbiol 54 (2004), 1991-1995; DOI  10.1099/ijs.0.02976-0
© 2004 International Union of Microbiological Societies

Geobacillus lituanicus sp. nov.

Nomeda Kuisiene, Juozas Raugalas and Donaldas Chitavichius

Department of Plant Physiology and Microbiology, Vilnius University, Chiurlionio 21/27, Vilnius LT-03101, Lithuania

Correspondence
Nomeda Kuisiene
nomeda.kuisiene{at}gf.vu.lt

Obligately thermophilic, aerobic, proteolytic, endospore-forming strain N-3T was isolated from a high-temperature oilfield in Lithuania. 16S rRNA gene sequence analysis placed this strain in genetic group 5 of the endospore formers. Geobacillus thermoleovorans appeared to be the closest phylogenetic neighbour (99·4 % sequence similarity). The G+C content of strain N-3T was 52·5 mol% and matched the range established for the genus Geobacillus. Studies of DNA–DNA relatedness and morphological and physiological analyses enabled strain N-3T to be described as a member of the genus Geobacillus, but could not assign this strain to any other known species of this genus. Results of this polyphasic study allowed characterization of strain N-3T as a novel species in the genus Geobacillus – Geobacillus lituanicus sp. nov. This species can be distinguished from G. thermoleovorans and Geobacillus stearothermophilus on the basis of 16S rRNA gene PCR-RFLP assays with the restriction endonucleases AluI, HaeIII and TaqI. The type strain of the novel species is N-3T (=DSM 15325T=VKM B-2294T).

Abbreviations: ARDRA, amplified rDNA restriction analysis; RSA, ribosomal spacer analysis

Published online ahead of print on 21 May 2004 as DOI 10.1099/ijs.0.02976-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain N-3T is AY044055.

Graphs showing the effects of temperature, pH and salinity on the growth rate of strain N-3T are available as supplementary material in IJSEM Online.




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D. R. Zeigler
Application of a recN sequence similarity analysis to the identification of species within the bacterial genus Geobacillus
Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1171 - 1179.
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