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Metschnikowia chrysoperlae sp. nov., Candida picachoensis sp. nov. and Candida pimensis sp. nov., isolated from the green lacewings Chrysoperla comanche and Chrysoperla carnea (Neuroptera: Chrysopidae)

¹ Dept of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
² Dept of Entomology, University of Arizona, Tucson, AZ 85721, USA

Correspondence
Sung-Oui Suh
ssuh{at}lsu.edu

Fourteen yeast isolates comprising three taxa were cultured from digestive tracts of adult Chrysoperla species (Neuroptera: Chrysopidae) and their eggs. The yeast taxa were distinguished based on an estimated molecular phylogeny, DNA sequences and traditional taxonomic criteria. The new yeasts are closely related to Metschnikowia pulcherrima but are sufficiently distinguished by sequence comparison of rRNA gene sequences to consider them as novel species. Here, three novel species are described and their relationships with other taxa in the Saccharomycetes are discussed. Metschnikowia chrysoperlae sp. nov. (type strain, NRRL Y-27615^T=CBS 9803^T) produced needle-shaped ascospores and was the only teleomorph found. Large numbers of chlamydospores similar to those observed in M. pulcherrima were also produced. The other two novel species are asexual yeasts, Candida picachoensis sp. nov. (type strain, NRRL Y-27607^T=CBS 9804^T) and Candida pimensis sp. nov. (type strain, NRRL Y-27619^T=CBS 9805^T), sister taxa of M. chrysoperlae and M. pulcherrima. A specialized relationship between yeasts and lacewing hosts may exist, because the yeasts were isolated consistently from lacewings only. Although M. chrysoperlae was isolated from eggs and adult lacewings, suggesting the possibility of vertical transmission, no yeast was isolated from larvae.

Published online ahead of print on 12 March 2004 as DOI 10.1099/ijs.0.63152-0.

The GenBank/EMBL/DDBJ accession numbers for the sequences determined in this study are AY452039–AY452052 for the LSU rRNA gene, AY452053–AY452055 for the SSU rRNA gene and AY494780–AY494785 for the ITS region and 5·8S rRNA gene.

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