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Int J Syst Evol Microbiol 54 (2004), 1747-1752; DOI  10.1099/ijs.0.63118-0
© 2004 International Union of Microbiological Societies

Desulfovibrio alaskensis sp. nov., a sulphate-reducing bacterium from a soured oil reservoir

Maria J. Feio1,{dagger}, Vitaly Zinkevich1, Iwona B. Beech1, Enric Llobet-Brossa2, Peter Eaton1,{ddagger}, Jürgen Schmitt3 and Jean Guezennec4

1 School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Rd, Portsmouth PO1 2DT, UK
2 Max-Planck-Institut für marine Mikrobiologie, Celsiusstr. 1, D-28359 Bremen, Germany
3 IWW, Rheinisch-Westfälisches Institut für Wasserchemie und Wassertechnologie, Moritzstr. 26, 45476 Mülheim/Ruhr, Germany
4 IFREMER, Centre de Brest, DRV/VP/BMH, BP 70, 29280 Plouzané, France

Correspondence
Iwona B. Beech
Iwona.Beech{at}port.ac.uk

A novel sulphate-reducing bacterium (Al1T) was recovered from a soured oil well in Purdu Bay, Alaska. Light and atomic force microscopy observations revealed that cells were Gram-negative, vibrio-shaped and motile by means of a single polar flagellum. The carbon and energy sources used by the isolate and the salinity, temperature and pH ranges facilitating its growth proved to be typical of a partial lactate-oxidizing, moderately halophilic, mesophilic, sulphate-reducing bacterium. Analysis of the fatty acid profile revealed that C18 : 0, isoC15 : 0 and isoC17 : 1{omega}7c were the predominant species. Fatty acid profile and complete 16S rRNA gene sequencing demonstrated the similarity between strain Al1T and members of the genus Desulfovibrio. The position of strain Al1T within the phylogenetic tree indicated that it clustered closely with Desulfovibrio vietnamensis DSM 10520T (98·9 % sequence similarity), a strain recovered from a similar habitat. However, whole-cell protein profiles, Fourier-transform infrared studies and DNA–DNA hybridization demonstrated that, in spite of the high level of 16S rRNA gene sequence similarity, there is sufficient dissimilarity at the DNA sequence level between D. vietnamensis DSM 10520T and strain Al1T (10·2 % similarity) to propose that strain Al1T belongs to a separate species within the genus Desulfovibrio. Based on the results obtained, the name Desulfovibrio alaskensis sp. nov. is therefore proposed, with Al1T (=NCIMB 13491T=DSM 16109T) as the type strain.

Abbreviations: AFM, atomic force microscopy; FT-IR spectroscopy, Fourier-transform infrared spectroscopy; SRB, sulphate-reducing bacterium

Published online ahead of print on 26 March 2004 as DOI 10.1099/ijs.0.63118-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Al1T is Y11984.

The fatty acid profile and an AFM image of strain Al1T, FT-IR spectra of various SRB and a dendrogram based on these spectra are available as supplementary material in IJSEM Online.

{dagger}Present address: IBVF – Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Av. Américo Vespucio 49, 41092 Sevilla, Spain.

{ddagger}Present address: IIQ – Instituto de Investigaciones Químicas, Centro de Investigaciones Científicas Isla de la Cartuja, Av. Américo Vespucio 49, 41092 Sevilla, Spain.




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