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Int J Syst Evol Microbiol 54 (2004), 1709-1715; DOI  10.1099/ijs.0.02885-0
© 2004 International Union of Microbiological Societies

Emended description of the species Lampropedia hyalina

Natuschka Lee1, Carmela Maria Cellamare2, Cristiano Bastianutti2, Ramon Rosselló-Mora3, Peter Kämpfer4, Wolfgang Ludwig1, Karl Heinz Schleifer1 and Loredana Stante2

1 Lehrstuhl für Mikrobiologie, TU München, Am Hochanger 4, D-85350 Freising, Germany
2 ENEA, Sezione Gestione Risorse Idriche, Via Martiri di Monte Sole, 4, I-40129 Bologna, Italy
3 Grup d'Oceanografia Interdisciplinari Institut Mediterrani d'Estudis Avançats (CSIC-UIB), C/Miquel Marqués 21, E-07190 Esporles Mallorca, Spain
4 Institut für Angewandte Mikrobiologie, Universität Giessen, Heinrich-Buff-Ring 26–32 (IFZ), D-35392 Giessen, Germany

Correspondence
Natuschka Lee
leen{at}mikro.biologie.tu-muenchen.de

Three Lampropedia hyalina strains from different habitats were compared by phenotypic, chemotaxonomic and molecular characteristics. All strains form coccoid cells and have been reported to grow as square tablets of eight to 64 cells. However, two of these strains (ATCC 11041T and ATCC 43383) have apparently lost this ability, and the third strain may temporarily lose this capacity under certain cultivation conditions. The three strains showed only minor differences in metabolic characteristics: the main significant physiological difference was the ability to accumulate polyphosphate under alternating anaerobic–aerobic conditions found for DSM 15336. The three strains showed high similarity in fatty acid composition and only slight differences in the G+C content (63–67 mol%) and DNA–DNA reassociation (90–95 % relatedness). Comparative 16S rRNA gene sequence analyses on these three strains and three Lampropedia hyalina 16S rRNA gene sequences deposited at NCBI showed that they are all very similar (>98·8 %) and that they form a distinct group among the ‘Betaproteobacteria’, showing between 94·6 and 93 % 16S rRNA gene similarity to members of various genera such as Acidovorax, Aquaspirillum, Brachymonas, Comamonas, Delftia and Xenophilus. Fluorescent in situ hybridization with oligonucleotide probes targeting betaproteobacteria on the 16S rRNA and 23S rRNA gene level further supported the conclusion that all investigated strains are members of the ‘Betaproteobacteria’. Two oligonucleotide probes were designed and successfully applied for culture-independent identification of Lampropedia hyalina by means of fluorescent in situ hybridization.

Abbreviations: GTA, green top agar

Published online ahead of print on 23 March 2004 as DOI 10.1099/ijs.0.02885-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Lampropedia hyalina strains DSM 15336, ATCC 11041T and ATCC 43383 are respectively AY291119, AY291120 and AY291121.






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