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Classification of the biphenyl- and polychlorinated biphenyl-degrading strain LB400^T and relatives as Burkholderia xenovorans sp. nov.

Johan Goris^1,, Paul De Vos¹, Jesús Caballero-Mellado², Joonhong Park³, Enevold Falsen⁴, John F. Quensen, III³, James M. Tiedje³ and Peter Vandamme¹

¹ Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Gent, Belgium
² P. de Ecología Molecular y Microbiana, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
³ Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
⁴ Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-413 46 Göteborg, Sweden

Correspondence
Johan Goris
gorisj{at}msu.edu

Strain LB400^T is the best-studied polychlorinated biphenyl (PCB) degrader. This organism has previously been allocated in the genus Burkholderia, since its 16S rRNA gene sequence shows 98·6 % sequence similarity to the type strains of Burkholderia graminis and Burkholderia terricola. A polyphasic study was undertaken to clarify the actual taxonomic position of this biotechnologically important organism and of two strains, one recovered from a blood culture vial and one from a coffee plant rhizosphere, both of which resembled strain LB400^T in their whole-cell protein patterns. DNA–DNA hybridization experiments revealed that the three strains represented a single novel species, for which the name Burkholderia xenovorans sp. nov. is proposed. Strains of this novel species can be differentiated phenotypically from nearly all other Burkholderia species by their inability to assimilate L-arabinose. The whole-cell fatty acid profile of B. xenovorans strains is consistent with their classification in the genus Burkholderia, with 18 : 17c, 16 : 17c, 16 : 0, 14 : 0 3OH, 16 : 0 3OH, 17 : 0 cyclo and 14 : 0 being the most abundant fatty acids. The G+C content of the species varies between 62·4 and 62·9 mol%. The type strain of B. xenovorans is LB400^T (=LMG 21463^T=CCUG 46959^T=NRRL B-18064^T).

Published online ahead of print on 9 July 2004 as DOI 10.1099/ijs.0.63101-0.

BOX-PCR patterns and ribotype profiles are available as supplementary material in IJSEM Online.

Present address: Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.

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