| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Veterinary Microbiology, Stigbøjlen 4, Royal Danish Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark
2 Institute of Veterinary Bacteriology, University of Bern, Laenggass-Strasse 122, CH-3012 Bern, Switzerland
Correspondence
Henrik Christensen
hech{at}kvl.dk
Phylogenies of housekeeping gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family Pasteurellaceae and knowledge of the evolutionary relationships of its members. Deduced partial protein sequences of the housekeeping genes atpD, infB and rpoB were compared in 28, 36 and 28 representative taxa of the Pasteurellaceae, respectively. The monophyly of representatives of the genus Gallibacterium was recognized by analysis of all housekeeping genes, while members of Mannheimia, Actinobacillus sensu stricto and the core group of Pasteurella sensu stricto formed monophyletic groups with two out of three housekeeping genes. Representatives of Mannheimia, Actinobacillus sensu stricto, [Haemophilus] ducreyi and [Pasteurella] trehalosi formed a monophyletic unit by analysis of all three housekeeping genes, which was in contrast to the 16S rRNA gene-derived phylogeny, where these taxa occurred at separate positions in the phylogenetic tree. Representatives of the Rodent, Avian and Aphrophilus–Haemophilus 16S rRNA gene groups were weakly supported by phylogenetic analysis of housekeeping genes. Phylogenies derived by comparison of the housekeeping genes diverged significantly from the 16S rRNA gene-derived phylogeny as evaluated by the likelihood ratio test. A low degree of congruence was also observed between the individual housekeeping gene-derived phylogenies. Estimates on speciation derived from 16S rRNA and housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the Pasteurellaceae. The phylogeny based on the housekeeping genes supported observed host associations between Mannheimia, Actinobacillus sensu stricto and [Pasteurella] trehalosi and animals with paired hooves.
The GenBank/EMBL/DDBJ accession number for the sequences obtained in this study are AY296207–AY296246 and AY314025–AY314043.
Details of strains analysed and sequence accession numbers are available as supplementary material in IJSEM Online.
This article has been cited by other articles:
|
|
 |
|
  U. Henssge, T. Do, D. R. Radford, S. C. Gilbert, D. Clark, and D. Beighton Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963 Int J Syst Evol Microbiol, March 1, 2009; 59(3): 509 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Martens, P. Dawyndt, R. Coopman, M. Gillis, P. De Vos, and A. Willems Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium) Int J Syst Evol Microbiol, January 1, 2008; 58(1): 200 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Naser, P. Dawyndt, B. Hoste, D. Gevers, K. Vandemeulebroecke, I. Cleenwerck, M. Vancanneyt, and J. Swings Identification of lactobacilli by pheS and rpoA gene sequence analyses Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2777 - 2789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Blackall, A. M. Bojesen, H. Christensen, and M. Bisgaard Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov. Int J Syst Evol Microbiol, April 1, 2007; 57(4): 666 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Angelos, P. Q. Spinks, L. M. Ball, and L. W. George Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis Int J Syst Evol Microbiol, April 1, 2007; 57(4): 789 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Martens, M. Delaere, R. Coopman, P. De Vos, M. Gillis, and A. Willems Multilocus sequence analysis of Ensifer and related taxa Int J Syst Evol Microbiol, March 1, 2007; 57(3): 489 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, P. Kuhnert, H.-J. Busse, W. C. Frederiksen, and M. Bisgaard Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae Int J Syst Evol Microbiol, January 1, 2007; 57(1): 166 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Olvera, M. Cerda-Cuellar, and V. Aragon Study of the population structure of Haemophilus parasuis by multilocus sequence typing Microbiology, December 1, 2006; 152(12): 3683 - 3690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. P Hanage, C. Fraser, and B. G Spratt Sequences, sequence clusters and bacterial species Phil Trans R Soc B, November 29, 2006; 361(1475): 1917 - 1927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gioia, X. Qin, H. Jiang, K. Clinkenbeard, R. Lo, Y. Liu, G. E. Fox, S. Yerrapragada, M. P. McLeod, T. Z. McNeill, et al. The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny. J. Bacteriol., October 1, 2006; 188(20): 7257 - 7266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kuhnert and B. M. Korczak Prediction of whole-genome DNA-DNA similarity, determination of G+C content and phylogenetic analysis within the family Pasteurellaceae by multilocus sequence analysis (MLSA). Microbiology, September 1, 2006; 152(Pt 9): 2537 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Korczak, R. Stieber, S. Emler, A. P. Burnens, J. Frey, and P. Kuhnert Genetic relatedness within the genus Campylobacter inferred from rpoB sequences. Int J Syst Evol Microbiol, May 1, 2006; 56(Pt 5): 937 - 945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Naser, F. L. Thompson, B. Hoste, D. Gevers, P. Dawyndt, M. Vancanneyt, and J. Swings Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes Microbiology, July 1, 2005; 151(7): 2141 - 2150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-L. Gautier, D. Dubois, F. Escande, J.-L. Avril, P. Trieu-Cuot, and O. Gaillot Rapid and Accurate Identification of Human Isolates of Pasteurella and Related Species by Sequencing the sodA Gene J. Clin. Microbiol., May 1, 2005; 43(5): 2307 - 2314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, M. Bisgaard, O. Angen, W. Frederiksen, and J. E. Olsen Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds J. Clin. Microbiol., January 1, 2005; 43(1): 259 - 270. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
