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Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov.

Karadeniz Technical University, Faculty of Arts and Sciences, Department of Biology, 61080 Trabzon, Turkey

Correspondence
Ali Osman Belduz
belduz{at}ktu.edu.tr

Two thermophilic bacilli were isolated from mud and water samples of the Ayder and Kestanbol hot springs in the provinces of Rize and Canakkale, respectively, in Turkey. Strains AB04^T and K4^T were sporulating, Gram-positive, rod-shaped bacteria. These isolates were moderately thermophilic (with an optimum temperature for growth of 50–55 °C), facultative anaerobes able to grow on a wide range of carbon sources including D-glucose, D-raffinose, D-sucrose, D-xylose, D-fructose, L-arabinose, maltose, D-mannose and D-mannitol. Analysis of the 16S rRNA gene sequences showed that these isolates resembled Anoxybacillus flavithermus DSM 2641^T and Anoxybacillus gonensis NCIMB 13933^T. DNA–DNA hybridization data revealed that thermophilic isolate AB04^T has only 51·2 % relatedness to A. flavithermus, 45·1 % relatedness to Anoxybacillus pushchinoensis and 68·6 % relatedness to A. gonensis. Thermophilic isolate K4^T showed only 60·4 % relatedness to A. flavithermus, 42·9 % relatedness to A. pushchinoensis and 38·5 % relatedness to A. gonensis. On the basis of the DNA–DNA hybridization data, isolates AB04^T and K4^T are not related to A. flavithermus DSM 2641^T, A. pushchinoensis DSM 12423^T or A. gonensis NCIMB 13933^T at the species level, but show relatedness to one another of 40·5 %. On the basis of the data presented, it is proposed that strains AB04^T (=NCIMB 13972^T=NCCB 100050^T) and K4^T (=NCIMB 13971^T=NCCB 100051^T) be designated as the type strains of Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov., respectively.

The GenBank accession numbers for the 16S rRNA gene sequences of strains AB04^T and K4^T are AF001963 and AY248711.

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				I. D. WAGNER and J. WIEGEL Diversity of Thermophilic Anaerobes Ann. N.Y. Acad. Sci., March 1, 2008; 1125(1): 1 - 43. [Abstract] [Full Text] [PDF]