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ena Korczak1
1 Institute of Veterinary Bacteriology, University of Bern, CH-3012 Bern, Switzerland
2 Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark
3 SmartGene GmbH, PSE-C EPFL-Ecublens, CH-1015 Lausanne, Switzerland
Correspondence
Peter Kuhnert
peter.kuhnert{at}vbi.unibe.ch
Sequences of the gene encoding the 
-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA–DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.
The GenBank accession numbers for the novel 16S rDNA and rpoB gene sequences of Pasteurellaceae species used in this study are given in Figs 1 and 2.
A similarity matrix of the rpoB sequences for all the strains investigated in this study is shown as supplementary data in IJSEM Online.
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