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Int J Syst Evol Microbiol 54 (2004), 1209-1212; DOI  10.1099/ijs.0.02939-0
© 2004 International Union of Microbiological Societies

Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam

Sun-Woo Nam1, Wonyong Kim1,2, Jongsik Chun1,3 and Michael Goodfellow1

1 School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
2 Department of Microbiology, Chung-Ang University College of Medicine, 221 Huksuk-dong, Dongjak-gu, Seoul 156-756, Republic of Korea
3 School of Biological Sciences, Seoul National University, 56-1 Shilim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea

Correspondence
Michael Goodfellow
m.goodfellow{at}ncl.ac.uk

The taxonomic position of two Tsukamurella strains isolated from activated sludge foam was clarified. The organisms, isolates JC85 and N1176T, were found to have chemical and morphological properties typical of members of the genus Tsukamurella. DNA–DNA relatedness studies showed that the strains formed a distinct genomic species that was most closely related to Tsukamurella spumae. The two isolates also share a range of phenotypic properties that distinguishes them from representatives of all species of Tsukamurella with validly published names. It is evident from the data that the two organisms should be classified as a novel Tsukamurella species, Tsukamurella pseudospumae sp. nov. The type strain is N1176T (=DSM 44118T=NCIMB 13963T).


The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of T. pseudospumae strains N1176T and JC85 are AY238513 and AY333425.




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