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Programa de Ecología Molecular y Microbiana, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Apdo. Postal 565-A, Cuernavaca, Morelos, México
Correspondence
Jesús Caballero-Mellado
jesuscab{at}cifn.unam.mx
It was shown recently that the genus Burkholderia is rich in N2-fixing bacteria that are associated with plants. A group of these diazotrophic isolates with identical or very similar 16S rDNA restriction patterns [designated amplified rDNA restriction analysis (ARDRA) genotypes 13, 14 and 15] was selected and a polyphasic taxonomic study was performed, which included new isolates that were recovered from rhizospheres, rhizoplanes or internal tissues of maize, sugarcane and coffee plants. Morphological, physiological and biochemical features, as well as multi-locus enzyme electrophoresis profiles and whole-cell protein patterns, of 20 strains were analysed. In addition, analysis of cellular fatty acid profiles, 16S rDNA sequence analysis and DNA–DNA reassociation experiments were performed with representative strains. The taxonomic data indicated that the strains analysed belong to a novel diazotrophic Burkholderia species, for which the name Burkholderia unamae sp. nov. is proposed. Strain MTl-641T (=ATCC BAA-744T=CIP 107921T), isolated from the rhizosphere of maize, was designated as the type strain. B. unamae was found as an endophyte of plants grown in regions with climates ranging from semi-hot subhumid to hot humid, but not from plants grown in regions with semi-hot or hot dry climates. Moreover, B. unamae was isolated from rhizospheres and plants growing in soils with pH values in the range 4·5–7·1, but not from soils with pH values higher than 7·5.
Published online ahead of print on 13 April 2004 as DOI 10.1099/ijs.0.02951-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences of strains MCo-762, MTl-641T and SCCu-23 are AY221955, AY221956 and AY221957, respectively.
A table of restriction patterns of B. unamae genotypes, a fuller phylogenetic tree and a dendrogram to illustrate the genetic relatedness of strains of B. unamae are available as supplementary material in IJSEM Online.
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