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Identification of the bacterial endosymbionts of the marine ciliate Euplotes magnicirratus (Ciliophora, Hypotrichia) and proposal of ‘Candidatus Devosia euplotis’

Dipartimento di Etologia, Ecologia, Evoluzione dell'Università di Pisa, Pisa, Italy

Correspondence
Claudia Vannini
c.vannini{at}deee.unipi.it

This paper reports the identification of bacterial endosymbionts that inhabit the cytoplasm of the marine ciliated protozoon Euplotes magnicirratus. Ultrastructural and full-cycle rRNA approaches were used to reveal the identity of these bacteria. Based on analysis of 16S rRNA gene sequences, evolutionary trees were constructed; these placed the endosymbiont in the genus Devosia in the -Proteobacteria. The validity of this finding was also shown by fluorescence in situ hybridization with a Devosia-specific oligonucleotide probe. Differences at the 16S rRNA gene level (which allowed the construction of a species-specific oligonucleotide probe) and the peculiar habitat indicate that the endosymbiont represents a novel species. As its cultivation has not been successful to date, the provisional name ‘Candidatus Devosia euplotis' is proposed. The species- and group-specific probes designed in this study could represent convenient tools for the detection of ‘Candidatus Devosia euplotis' and Devosia-like bacteria in the environment.

Published online ahead of print on 9 January 2004 as DOI 10.1099/ijs.0.02759-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of ‘Candidatus Devosia euplotis' from Euplotes magnicirratus strains LIV5, CO and CAMP4.4 are AJ548825, AJ548823 and AJ548824, respectively. The accession number for the 16S rRNA gene sequence of Devosia riboflavina DSM 7230^T is AJ549086. The accession numbers for the 18S rRNA gene sequences of Euplotes magnicirratus strains LIV5 and CAMP4.4 are AJ549209 and AJ549210, respectively.

A complete similarity matrix and a colour version of Fig. 3 are available as supplementary material in IJSEM Online.

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