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Int J Syst Evol Microbiol 54 (2004), 1111-1116; DOI  10.1099/ijs.0.02985-0
© 2004 International Union of Microbiological Societies

Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea

Jung-Hoon Yoon1, In-Gi Kim1, Tae-Kwang Oh1 and Yong-Ha Park1,2

1 Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea
2 National Research Laboratory of Molecular Ecosystematics, Institute of Probionic, Probionic Corporation, Bio-venture Center, KRIBB, PO Box 115, Yusong, Taejon, Korea

Correspondence
Jung-Hoon Yoon
jhyoon{at}kribb.re.kr

A Gram-negative, rod-shaped, non-spore-forming, slightly halophilic bacterium (strain TF-17T) was isolated from an intertidal sediment from the Yellow Sea, Korea. Pigment of strain TF-17T was similar to that of Microbulbifer elongatus, but different from those of Microbulbifer hydrolyticus and Microbulbifer salipaludis. Strain TF-17T was distinguishable from M. elongatus by some phenotypic properties, including motility, optimal growth temperature and others. Phylogenetic analysis based on 16S rDNA sequences showed that strain TF-17T clustered with the type strains of the three Microbulbifer species with validly published names. Strain TF-17T exhibited 16S rDNA sequence similarity levels of 95·1–95·7 % to the type strains of the three Microbulbifer species. The predominant respiratory lipoquinone found in strain TF-17T was ubiquinone-8. The major fatty acid was iso-C15 : 0 and significant amounts of iso-C11 : 0 3-OH and iso-C17 : 1{omega}9c were also present. The DNA G+C content of strain TF-17T was 59·9 mol%. Levels of DNA–DNA relatedness between strain TF-17T and the type strains of the three Microbulbifer species were in the range 10·0–13·0 %. On the basis of phenotypic and phylogenetic data and genotypic distinctiveness, strain TF-17T (=KCCM 41774T=JCM 12187T) is proposed as the type strain of a novel species of the genus Microbulbifer, Microbulbifer maritimus sp. nov.


Abbreviations: TEM, transmission electron microscopy

Published online ahead of print on 23 January 2004 as DOI 10.1099/ijs.0.02985-0.

The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain TF-17T is AY377986.




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