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Int J Syst Evol Microbiol 54 (2004), 1095-1100; DOI  10.1099/ijs.0.02887-0
© 2004 International Union of Microbiological Societies

Methanotorris formicicus sp. nov., a novel extremely thermophilic, methane-producing archaeon isolated from a black smoker chimney in the Central Indian Ridge

Ken Takai1, Ken H. Nealson1,2 and Koki Horikoshi1

1 Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
2 Department of Earth Sciences, University of Southern California, 3651 Trousdale Pkwy, Los Angeles, CA 90089-0740, USA

Correspondence
Ken Takai
kent{at}jamstec.go.jp

A novel extremely thermophilic, methane-producing archaeon was isolated from a black smoker chimney at the Kairei field in the Central Indian Ridge. Cells of this isolate were irregular cocci with several flagella; motility was not observed. Growth was observed between 55 and 83 °C (optimum of 75 °C; 30 min doubling time) and between pH 6·0 and 8·5 (optimum of pH 6·7). The isolate was a strictly anaerobic, methanogenic autotroph capable of using hydrogen and carbon dioxide as sole energy and carbon sources. Formate was utilized as an alternative energy source. The G+C content of the genomic DNA was 33·3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was most closely related to Methanotorris igneus strain Kol 5T. The isolate, however, could be genetically differentiated from this species by DNA–DNA hybridization analysis and on the basis of its physiological properties. The name Methanotorris formicicus sp. nov. is proposed for this isolate; the type strain is Mc-S-70T (=JCM 11930T=ATCC BAA-687T).

Abbreviations: CIR, Central Indian Ridge; MAR, Mid-Atlantic Ridge

Published online ahead of print on 12 December 2003 as DOI 10.1099/ijs.0.02887-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Mc-S-70T is AB100884.

Graphs showing the effects of temperature, pH and NaCl concentration on growth of Methanotorris formicicus are available as supplementary figures A, B and C, respectively, in IJSEM Online.




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