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State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China
Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn
A strict anaerobe (strain GB8-1T) that degraded straight-chain fatty acids with C4–C18 in syntrophic association with methanogens was isolated from an up-flow anaerobic sludge blanket reactor treating beer wastewater. Strain GB8-1T degraded 1 mol butyrate into about 2 mol acetate and 1 mol (presumably) H2 in co-culture with a methanogen. Neither branched-chain fatty acids nor benzoate could be degraded. Strain GB8-1T could grow on crotonate in pure culture and converted 1 mol crotonate to 0·5 mol butyrate and 1 mol acetate. Generation time was about 11 h when grown on crotonate at 37 °C. Fumarate, sulfate, thiosulfate, sulfur and nitrate could not serve as electron acceptors for strain GB8-1T to degrade butyrate. Cells of strain GB8-1T were curved rods with Gram-negative cell walls; no spores were observed. The DNA G+C content was 46·6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GB8-1T was related most closely to the fatty acid-oxidizing, syntrophic bacterium Syntrophomonas sapovorans DSM 3441T; however, the relationship was not very close (95·4 % sequence similarity). Some phenotypic features also differentiated strain GB8-1T from Syntrophomonas sapovorans DSM 3441T. Therefore, a novel species, Syntrophomonas curvata sp. nov., is proposed. The type strain is GB8-1T (=CGMCC 1.5010T=DSM 15682T).
Published online ahead of print on 9 January 2004 as DOI 10.1099/ijs.0.02903-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Syntrophomonas curvata strain GB8-1T is AY290767.
Transmission electron micrographs of strain GB8-1T are available as supplementary material in IJSEM Online.
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