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Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.

Célia M. Manaia¹, Balbina Nogales^2,3,, Norbert Weiss⁴ and Olga C. Nunes⁵

¹ Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal
² Division of Microbiology, GBF – German Research Centre for Biotechnology, Braunschweig, Germany
³ Area de Microbiologia, Universitat de les Illes Balears, Palma de Mallorca, Spain
⁴ DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany
⁵ LEPAE – Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, R. Dr Roberto Frias, 4200-465 Porto, Portugal

Correspondence
Olga C. Nunes
opnunes{at}fe.up.pt

A Gram-positive, molinate-degrading bacterium, strain ON4^T (=DSM 13485^T=LMG 21909^T), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10–41 °C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on D-ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent in the family Microbacteriaceae, showing the highest 16S rDNA similarity (95 %) to members of the genus Curtobacterium and ‘Brevibacterium helvolum’ DSM 20419 (=ATCC 13715). The latter was reported to have the cell-wall peptidoglycan type B2 and the major menaquinone MK-9, which are typical of Clavibacter, but it is clearly separated from this genus at the phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously described genera and their distinctive phenotypic characteristics, it is proposed that strains ON4^T and ‘B. helvolum’ DSM 20419 be classified as two novel genera and species, with the respective names Gulosibacter molinativorax gen. nov., sp. nov. and Pseudoclavibater helvolus gen. nov., sp. nov.

Published online ahead of print on 28 November 2003 as DOI 10.1099/ijs.0.02851-0.

The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of isolate ON4^T is AJ306835.

Micrographs of cells of the novel species and details of fatty acid compositions are available as supplementary material in IJSEM Online.

Present address: Area de Microbiologia, Universitat de les Illes Balears, Palma de Mallorca, Spain.

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