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1 Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, Université de la Méditerrannée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
2 Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Leahurst, Neston CH64 7TE, UK
3 Respiratory and Systemic Infections Laboratory, Central Public Health Laboratory, Public Health Laboratory Service, London NW9 5HT, UK
4 Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
Correspondence
Didier Raoult
raoult{at}medecine.univ-mrs.fr
A Legionella-like amoebal pathogen (LLAP), formerly named LLAP12T, was characterized on the basis of microscopic appearance, staining characteristics, growth in Acanthamoeba polyphaga at different temperatures, DNA G+C content, serological cross-reactivity and 16S rRNA and macrophage infectivity potentiator (mip) gene sequence analysis. LLAP12T was found to be a motile, Gram-negative bacterium that grew within cytoplasmic vacuoles in infected amoebae. The infecting bacteria induced lysis of their amoebal hosts and time taken to do so was dependent on incubation temperature. Recovery of LLAP12T from amoebae onto axenic media could not be achieved. Phylogenetic analysis of LLAP12T, based on 16S rRNA and mip gene sequence analysis, indicated that it lay within the radiation of the Legionellaceae and that it clustered specifically with Legionella lytica and Legionella rowbothamii. The divergence observed between LLAP12T and these two species was of a degree equal to, or greater than, that observed between other members of the family. In support of this delineation, LLAP12T was found not to cross-react serologically with any other Legionella species. The mip and 16S rRNA gene sequence-based analyses also indicated that LLAP12T was related very closely to two other previously identified LLAP isolates, LLAP4 and LLAP11. Taken together, these results support the proposal of LLAP12T as the type strain of Legionella drancourtii sp. nov.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of L. drancourtii LLAP12T is X97366 and that for the mip gene sequence is AF407571.
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