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1 Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium
2 Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium
3 Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109-0646, USA
4 Cardiff School of Biosciences, PO Box 915, Cardiff University, Cardiff CF10 3TL, UK
Correspondence
Peter Vandamme
Peter.Vandammme{at}UGent.be
Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943T (=CCUG 47727T) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.
The GenBank/EMBL/DDBJ accession numbers for the recA genes of strains LMG 21820 and LMG 21443 are AY324807 and AY324808.
Details of the 18 isolates of B. dolosa sp. nov. included in this study are available as supplementary material in IJSEM Online.
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