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1 National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada R3E 3R2
2 Laboratoire de santé publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada
3 Royal University Hospital, Saskatoon, Saskatchewan, Canada
4 Division of Respiratory Medicine, Department of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
5 Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
Correspondence
C. Y. Turenne
cturenne{at}hc-sc.gc.ca
A pigmented, slowly growing Mycobacterium avium complex AccuProbe-positive organism was isolated from the sputum and pleural fluid of a 72-year-old female with bronchiectasis. The unusual morphology of the organism prompted further identification by 16S rRNA gene sequencing, revealing a perfect identity with previously uncharacterized strain Mycobacterium sp. MCRO 8 (GenBank accession no. X93034), with the closest established species by 16S rDNA analysis being Mycobacterium interjectum. HPLC of the organism corresponded to previously obtained patterns identified as M. interjectum-like and, upon sequence evaluation of a selection of strains with a similar profile, more were subsequently identified as MCRO 8. A total of 16 strains isolated from human respiratory samples were evaluated in the characterization of this novel species, for which the name Mycobacterium saskatchewanense sp. nov. is proposed. The type strain is strain 00-250T (=ATCC BAA-544T=DSM 44616T=CIP 108114T).
Published online ahead of print on 31 October 2003 as DOI 10.1099/ijs.0.02739-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene, ITS1 and partial hsp65 gene sequences of strains 00-250T and MB54784 are AY208856–AY208859.
Details of the strains analysed in this study, photos of colonies and an ITS1 alignment are available as supplementary material in IJSEM Online.
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