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Int J Syst Evol Microbiol 54 (2004), 593-598; DOI  10.1099/ijs.0.02941-0
© 2004 International Union of Microbiological Societies

Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase {beta}-subunit gene (rpoB) sequences

Bum-Joon Kim1, Chang-Jin Kim2, Jongsik Chun3, Young-Hwan Koh4, Sueng-Hyun Lee5, Jin-Won Hyun6, Chang-Yong Cha1 and Yoon-Hoh Kook1

1 Department of Microbiology, College of Medicine, Seoul National University, Seoul 110-799, Korea
2 Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon 305-600, Korea
3 School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
4 Department of Food Science and Engineering, Cheju National University, Jeju 690-756, Korea
5 Department of Microbiology, College of Medicine, Konkuk University, Chungju 380-230, Korea
6 Department of Biochemistry, College of Medicine, Cheju National University, Jeju 690-756, Korea

Correspondence
Bum-Joon Kim
kbumjoon{at}snu.ac.kr

The RNA polymerase {beta}-subunit genes (rpoB) of 67 Streptomyces strains, representing 57 species, five Kitasatospora strains and Micromonospora echinospora KCTC 9549 were partially sequenced using a pair of rpoB PCR primers. Among the streptomycetes, 99·7–100 % similarity within the same species and 90·2–99·3 % similarity at the interspecific level were observed by analysis of the determined rpoB sequences. The topology of the phylogenetic tree based on rpoB sequences was similar to that of 16S rDNA. The five Kitasatospora strains formed a stable monophyletic clade and a sister group to the clade comprising all Streptomyces species. Although there were several discrepancies in the details, considerable agreement was found between the results of rpoB analysis and those of numerical phenetic classification. This study demonstrates that analysis of rpoB can be used as an alternative genetic method in parallel to conventional taxonomic methods, including numerical phenetic and 16S rDNA analyses, for the phylogenetic analyses of the genera Streptomyces and Kitasatospora.

Published online ahead of print on 21 November 2003 as DOI 10.1099/ijs.0.02941-0.

The GenBank accession numbers for the partial RNA polymerase gene sequences reported in this study are AY280726–AY280797 and AY281747.

Details of the 73 strains included in this study are available as supplementary material in IJSEM Online.




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Y. Guo, W. Zheng, X. Rong, and Y. Huang
A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematics
Int J Syst Evol Microbiol, January 1, 2008; 58(1): 149 - 159.
[Abstract] [Full Text] [PDF]


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