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Taxonomy of the canine Mollicutes by 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequence comparison

Victoria J. Chalker and Joe Brownlie

Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire, AL9 7TA, UK

Correspondence
Victoria J. Chalker
vicki.chalker{at}hpa.org.uk

The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S rRNA intergenic spacer (IGS) region sequences. The nucleotide sequences of the 16S rRNA gene of two untyped mycoplasmas and the IGS region of 11 Mycoplasma species were determined and used for phylogenetic analysis. The two untyped Mycoplasma strains, HRC 689 and VJC 358, were found to be distinct from all known canine mycoplasmas and all published mycoplasma 16S rRNA gene sequences.

Published online ahead of print on 3 October 2003 as DOI 10.1099/ijs.0.02869-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequences included in this study are: Mycoplasma sp. strain HRC 689, AF527624; Mycoplasma sp. strain VJC 358, AY246564; Mycoplasma cynos, AF538682; Mycoplasma molare, AF538683; Mycoplasma opalescens, AF538961; Mycoplasma spumans, AF538684. In addition, the following 16S/23S rRNA intergenic spacer region sequences were determined: Mycoplasma arginini, AF443604; Mycoplasma canis, AF443605; M. cynos, AF443606; Mycoplasma edwardii, AF443607; Mycoplasma felis, AF443608; Mycoplasma gateae, AF443609; Mycoplasma maculosum, AF443610; M. molare, AF443611; M. opalescens, AF443612.

A figure showing growth of Mycoplasma sp. strain VJC 358 on solid medium is available as supplementary material in IJSEM Online.

Present address: Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK.

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