| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany
2 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany
3 Abteilung für Mikrobielle Ökologie, Institut für Ökologie und Naturschutz (IECB), Universität Wien, Althanstr. 14, A-1090 Wien, Austria
Correspondence
Stefan Spring
ssp{at}dsmz.de
Strain K11T was isolated from activated sludge of a municipal wastewater-treatment plant. Phylogenetic analysis of the 16S rRNA gene sequence revealed that it represents a distinct line of descent within the Comamonadaceae. The novel strain was a Gram-negative, catalase- and oxidase-positive, non-motile, straight to slightly curved rod. Polyhydroxyalkanoate granules were stored intracellularly as reserve material. Colonies on agar plates were small, regular and characterized by a water-insoluble yellow pigment. Unbranched fatty acids 16 : 1
7c, 16 : 0 and 18 : 17c dominated the cellular fatty acid pattern and ubiquinone-8 (Q-8) was the major component of the respiratory lipoquinones, both traits typical of members of the Comamonadaceae. A distinguishing characteristic was the presence of the two hydroxy fatty acids 10 : 0 3-OH and 12 : 0 2-OH, each in significant amounts. The G+C content of the DNA was 59 mol%. Strain K11T was capable of aerobic chemolithoheterotrophic growth using thiosulfate as an additional substrate, but could not grow autotrophically with thiosulfate or hydrogen. Facultative anaerobic growth was possible with nitrate and nitrite as electron acceptors, but not with ferric iron, sulfate or by fermentation. The sole end product of denitrification was N2O; nitrite accumulated only transiently in small amounts. Based upon phylogenetic and phenotypic evidence, it is proposed to establish the novel taxon Ottowia thiooxydans gen. nov., sp. nov., represented by the type strain K11T (=DSM 14619T=JCM 11629T). Aquaspirillum gracile was among the phylogenetically most closely related species to strain K11T. This species has been wrongly classified, and it is proposed to reclassify it as Hylemonella gracilis gen. nov., comb. nov. The type strain is ATCC 19624T (=DSM 9158T).
The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain K11T is AJ537466.
A 16S rDNA-based neighbour-joining tree and characteristics that differentiate the novel taxon from some genera of the Comamonadaceae are available as supplementary material in IJSEM Online.
This article has been cited by other articles:
|
|
 |
|
  S. H. Ryu, D. S. Lee, M. Park, Q. Wang, H. H. Jang, W. Park, and C. O. Jeon Caenimonas koreensis gen. nov., sp. nov., isolated from activated sludge Int J Syst Evol Microbiol, May 1, 2008; 58(5): 1064 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Yoon, S.-J. Kang, S. H. Ryu, C. O. Jeon, and T.-K. Oh Hydrogenophaga bisanensis sp. nov., isolated from wastewater of a textile dye works Int J Syst Evol Microbiol, February 1, 2008; 58(2): 393 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kampfer, K. Thummes, H.-I Chu, C.-C. Tan, A. B. Arun, W.-M. Chen, W.-A. Lai, F.-T. Shen, P. D. Rekha, and C.-C. Young Pseudacidovorax intermedius gen. nov., sp. nov., a novel nitrogen-fixing betaproteobacterium isolated from soil Int J Syst Evol Microbiol, February 1, 2008; 58(2): 491 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Yoon, S.-J. Kang, S. Park, S.-Y. Lee, and T.-K. Oh Reclassification of Aquaspirillum itersonii and Aquaspirillum peregrinum as Novispirillum itersonii gen. nov., comb. nov. and Insolitispirillum peregrinum gen. nov., comb. nov. Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2830 - 2835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Chung, S. H. Ryu, M. Park, Y. Jeon, Y. R. Chung, and C. O. Jeon Hydrogenophaga caeni sp. nov., isolated from activated sludge Int J Syst Evol Microbiol, May 1, 2007; 57(5): 1126 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Grabovich, E. Gavrish, J. Kuever, A. M. Lysenko, D. Podkopaeva, and G. Dubinina Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov., and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov. Int J Syst Evol Microbiol, March 1, 2006; 56(Pt 3): 569 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Spring, M. Wagner, P. Schumann, and P. Kampfer Malikia granosa gen. nov., sp. nov., a novel polyhydroxyalkanoate- and polyphosphate-accumulating bacterium isolated from activated sludge, and reclassification of Pseudomonas spinosa as Malikia spinosa comb. nov. Int J Syst Evol Microbiol, March 1, 2005; 55(2): 621 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kampfer, R. Schulze, U. Jackel, K. A. Malik, R. Amann, and S. Spring Hydrogenophaga defluvii sp. nov. and Hydrogenophaga atypica sp. nov., isolated from activated sludge Int J Syst Evol Microbiol, January 1, 2005; 55(1): 341 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Lee, C. M. Cellamare, C. Bastianutti, R. Rossello-Mora, P. Kampfer, W. Ludwig, K. H. Schleifer, and L. Stante Emended description of the species Lampropedia hyalina Int J Syst Evol Microbiol, September 1, 2004; 54(5): 1709 - 1715. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
