| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria
2 Cientific Titular CSIC, GOI, IMEDEA (CSIC-UIB), C/Miquel Marqués 21, E-07190 Esporles, Mallorca, Spain
3 Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria
Correspondence
Hans-Jürgen Busse
hans-juergen.busse{at}vu-wien.ac.at
Four coagulase-negative, novobiocin-resistant cocci, designated CW1T, PM34, MM3 and RW78, were isolated from the respiratory tract of goats kept in the Himalayan region. The four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after SDS-PAGE and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (ERIC)-PCR. Strain CW1T showed highest 16S rDNA sequence similarities to Staphylococcus cohnii subsp. urealyticus ATCC 49330T, Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305T, S. cohnii subsp. cohnii ATCC 29974T, Staphylococcus arlettae ATCC 43957T, Staphylococcus gallinarum ATCC 35539T, Staphylococcus succinus ATCC 700337T and Staphylococcus xylosus ATCC 29971T (99·0, 98·8, 98·8, 98·4, 98·2, 98·1 and 98·1 %, respectively), indicating its classification within the genus Staphylococcus. The polar lipid composition, fatty acid profiles, quinone systems and diagnostic cell-wall diamino acid were in agreement with the characteristics of the genus Staphylococcus. DNA–DNA hybridization with closely related Staphylococcus species suggested that strain CW1T represents an as-yet unrecognized species. Based on these results, a novel species of the genus Staphylococcus is described, Staphylococcus nepalensis sp. nov. The type strain is CW1T (=DSM 15150T=CCM 7045T) and the most dissimilar strain is PM34 (=DSM 15151=CCM 7046).
The EMBL accession number for the 16S rDNA sequence of strain CW1T is AJ517414.
SDS-PAGE profiles of whole-cell lysates of the novel isolates and reference Staphylococcus species are available as supplementary material in IJSEM Online.
This article has been cited by other articles:
|
|
 |
|
  B. Ghebremedhin, F. Layer, W. Konig, and B. Konig Genetic Classification and Distinguishing of Staphylococcus Species Based on Different Partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf Gene Sequences J. Clin. Microbiol., March 1, 2008; 46(3): 1019 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Novakova, I. Sedlacek, R. Pantucek, V. Stetina, P. Svec, and P. Petras Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens. J. Med. Microbiol., May 1, 2006; 55(Pt 5): 523 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Pantucek, I. Sedlacek, P. Petras, D. Koukalova, P. Svec, V. Stetina, M. Vancanneyt, L. Chrastinova, J. Vokurkova, V. Ruzickova, et al. Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1953 - 1958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Giammarinaro, S. Leroy, J.-P. Chacornac, J. Delmas, and R. Talon Development of a New Oligonucleotide Array To Identify Staphylococcal Strains at Species Level J. Clin. Microbiol., August 1, 2005; 43(8): 3673 - 3680. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
