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1 Institute for Biological Resources and Function, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
2 Laboratory of Electron Microscopy, School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan
Correspondence
Isao Yumoto
i.yumoto{at}aist.go.jp
A facultatively psychrophilic bacterium, strain MD17T, which hydrolyses lipids at 5 °C, was isolated from the Monbetsu coast of the Okhotsk Sea in Hokkaido, Japan, when ice carried by the cold current came to the area. The isolate is an aerobic, non-motile coccobacillus that reduces nitrate to nitrite and hydrolyses Tweens 20, 40, 60 and 80, but not gelatin, DNA or alginic acid. The isolate grows at 0 °C, but not at temperatures higher than 36 °C; its optimum growth temperature is 25 °C. It grows in the presence of 0–10 % NaCl. Its major isoprenoid quinone is ubiquinone-8 (Q-8) and its DNA G+C content is 46·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MD17T is closely related to Psychrobacter glacincola DSM 12194T (99·0 % similarity) and Psychrobacter immobilis DSM 7229T (98·7 % similarity). DNA–DNA hybridization revealed 45·9 % relatedness between strain MD17T and P. immobilis ATCC 43116T and 33·4 % between strain MD17T and P. glacincola ATCC 700754T. Based on physiological and biochemical characteristics, phylogenetic position (as determined by 16S rRNA gene sequence analysis) and DNA–DNA relatedness, it is concluded that the isolate should be designated as a novel species, for which the name Psychrobacter okhotskensis sp. nov. is proposed. The type strain is MD17T (=NCIMB 13931T=JCM 11840T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MD17T is AB094794.
A table showing DNA–DNA relatedness among strains examined in this study and micrographs showing the morphology of strain MD17T are available as supplementary material in IJSEM Online.
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