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1 Departamento de Producción Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Apartado 257, 37071 Salamanca, Spain
2 Departamento de Microbiología y Genética, Universidad de Salamanca, 37007 Salamanca, Spain
3 Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
Correspondence
José-Mariano Igual
igual{at}usal.es
Several bacterial strains were isolated from root nodules of Phaseolus vulgaris plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the 
-Proteobacteria and that the closest related genus is Herbaspirillum. The DNA G+C content ranged from 57·9 to 61·9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, meso-inositol, meso-erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus Herbaspirillum, and the name Herbaspirillum lusitanum sp. nov. is proposed. The type strain is P6-12T (=LMG 21710T=CECT 5661T).
Published online ahead of print on 30 May 2003 as DOI 10.1099/ijs.0.02677-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Herbaspirillum lusitanum P6-12T is AF543312.
Details of DNA G+C contents, levels of DNA–DNA relatedness and pellicle formation by the novel isolates, transmission electron micrographs of cells, a 16S rDNA-based phylogenetic tree and RFLP and TP-RAPD patterns of the novel isolates are available as supplementary material in IJSEM Online.
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